Abstract

Restrictive anorexia nervosa is associated with reduced eating and severe body weight loss leading to a cachectic state. Hypothalamus plays a major role in the regulation of food intake and energy homeostasis. In the present study, alterations of hypothalamic proteome and particularly of proteins involved in energy and mitochondrial metabolism have been observed in female activity-based anorexia (ABA) mice that exhibited a reduced food intake and a severe weight loss. In the hypothalamus, mitochondrial dynamic was also modified during ABA with an increase of fission without modification of fusion. In addition, increased dynamin-1, and LC3II/LC3I ratio signed an activation of autophagy while protein synthesis was increased. In conclusion, proteomic analysis revealed an adaptive hypothalamic protein response in ABA female mice with both altered mitochondrial response and activated autophagy.

Details

Title
Alterations of proteome, mitochondrial dynamic and autophagy in the hypothalamus during activity-based anorexia
Author
Nobis, Séverine 1 ; Goichon, Alexis 1 ; Achamrah, Najate 2 ; Guérin, Charlène 1 ; Azhar, Saida 1 ; Chan, Philippe 3 ; Morin, Aline 1 ; Bôle-Feysot, Christine 1 ; do Rego, Jean Claude 4 ; Vaudry, David 5 ; Déchelotte, Pierre 2   VIAFID ORCID Logo  ; Belmonte, Liliana 2 ; Coëffier, Moïse 2 

1  Normandie University, UNIROUEN, INSERM Unit 1073, Rouen, France; Normandie University, UNIROUEN, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France 
2  Normandie University, UNIROUEN, INSERM Unit 1073, Rouen, France; Normandie University, UNIROUEN, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France; Rouen University Hospital, Nutrition Department, Rouen, France 
3  Normandie University, UNIROUEN, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France; Normandie University, UNIROUEN, Platform in proteomics PISSARO, Rouen, France 
4  Normandie University, UNIROUEN, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France; Normandie University, UNIROUEN, Animal Behaviour Platform SCAC, Rouen, France 
5  Normandie University, UNIROUEN, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France; Normandie University, UNIROUEN, Platform in proteomics PISSARO, Rouen, France; Normandie University, UNIROUEN, INSERM Unit 1239, Mont-Saint-Aignan, France 
Pages
1-15
Publication year
2018
Publication date
May 2018
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-018-25548-9
ProQuest document ID
2036457854
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.