Abstract

Fluorescence in situ hybridization (FISH) is used to visualize the distribution of DNA elements within a genome. Conventional methods for FISH take 1–2 days. Here, we developed a simplified, rapid FISH technique using pre-labeled oligonucleotide probes (PLOPs) and tested the procedure using 18 PLOPs from 45S and 5S rDNA, Arabidopsis-type telomere, and newly-identified Panax ginseng-specific tandem repeats. The 16 developed rDNA PLOPs can be universally applied to plants and animals. The telomere PLOPs can be utilized in most plants with Arabidopsis-type telomeres. The ginseng-specific PLOP can be used to distinguish P. ginseng from related Panax species. Differential labeling of PLOPs allowed us to simultaneously visualize different target loci while reducing the FISH hybridization time from ~16 h to 5 min. PLOP-FISH is efficient, reliable, and rapid, making it ideal for routine analysis, especially of newly sequenced genomes using either universal or specific targets, such as novel tandem repeats identified from whole-genome sequencing data.

Details

Title
Rapid and Efficient FISH using Pre-Labeled Oligomer Probes
Author
Nomar Espinosa Waminal 1 ; Remnyl Joyce Pellerin 2 ; Nam-Soo, Kim 3   VIAFID ORCID Logo  ; Jayakodi, Murukarthick 4 ; Jee Young Park 4 ; Tae-Jin, Yang 4   VIAFID ORCID Logo  ; Kim, Hyun Hee 2 

1  Chromosome Research Institute, Department of Life Science, Sahmyook University, Seoul, Korea; Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea 
2  Chromosome Research Institute, Department of Life Science, Sahmyook University, Seoul, Korea 
3  Department of Molecular Biosciences, Kangwon National University, Chuncheon, Korea 
4  Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea 
Pages
1-10
Publication year
2018
Publication date
May 2018
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-018-26667-z
ProQuest document ID
2046589377
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.