A less selfish view of genome size evolution in maize

Abstract

Genome size varies by many orders of magnitude across plants and animals, but resolving the most important evolutionary forces driving this variation remains challenging. Since eukaryotic genome size variation is not associated with complexity, genetic drift of the amount of noncoding DNA could dominate, implicating population and species history as key drivers of shifts in DNA content. Alternatively, directional selection could be acting on DNA content, but if so, it has not been resolved which level of selection is most important. Since the predominant component of many eukaryotic genomes is comprised of selfish genetic elements such as transposable elements (TEs) and regions subject to meiotic drive, factors that influence their differential success across populations and species could account for much of the variation in genome size. [6] add to this rich literature by using a quantitative genetics framework to conduct a test of local adaptation, recognizing that genome size is a trait governed by an immense number of small-effect loci. [...]it can be thought of as a quantitative trait under complete genetic control, since the variation in genome size is expected to be a simple function of the net number of insertion and deletion alleles individuals have across the genome. Conversely, other selective pressures on selfish genetic elements may provide an additional source of genome size variation in maize populations beyond the effects of flowering time. Since chromosomal knobs are known to have severe fitness costs, the extent of these costs may well depend on the environment [22], possibly explaining the disproportionate effect of altitude on knobs when genome size is controlled for in this study.

About the Authors:

Julia M. Kreiner

Affiliation: Department of Ecology & Evolutionary Biology, University of Toronto, Toronto, Ontario, Canada

ORCID http://orcid.org/0000-0002-8593-1394

Stephen I. Wright

* E-mail: [email protected]

Affiliation: Department of Ecology & Evolutionary Biology, University of Toronto, Toronto, Ontario, Canada

Citation: Kreiner JM, Wright SI (2018) A less selfish view of genome size evolution in maize. PLoS Genet 14(5): e1007249. https://doi.org/10.1371/journal.pgen.1007249

Editor: Gregory P. Copenhaver, The University of North Carolina at Chapel Hill, UNITED STATES

Published: May 10, 2018

Copyright: © 2018 Kreiner, Wright. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Funding: SIW was supported by an NSERC DIscovery Grant, and an E.W.R. Steacie Fellowship. JMK was supported by an NSERC PGS-D postgraduate fellowship (http://www.nserc-crsng.gc.ca/index_eng.asp). The funders had no role in the preparation of the article.

Competing interests: The authors have declared that no competing interests exist.

Genome size varies by many orders of magnitude across plants and animals, but resolving the most important evolutionary forces driving this variation remains challenging. Since eukaryotic genome size variation is not associated with complexity, genetic drift of the amount of noncoding DNA could dominate, implicating population and species history as key drivers of shifts in DNA content. Alternatively, directional selection could be acting on DNA content, but if so, it has not been resolved which level of selection is most important. Since the predominant component of many eukaryotic genomes is comprised of selfish genetic elements such as transposable elements (TEs) and regions subject to meiotic drive, factors that influence their differential success across populations and species could account for much of the variation in genome size. However, DNA content can also have important effects on organismal phenotype that could be under directional selection. Genome size may often be an important determinant of cell size and division rate and the subject of selection via its effects on developmental and metabolic phenotypes [1,2]. Supporting this view, correlations of genome size with invasive potential and growth rate [3], regional abundance and seed size [4], and even metabolic intensity in flying birds [5], may suggest an adaptive role. In this issue, Bilinski et al. [6] present evidence for this organismal adaptation view of genome size evolution by providing an explicit test for natural selection on genome size and repeat abundance across multiple altitudinal clines in maize and its wild relatives and identifying the underlying physiological mechanism.

Bilinski et al. [6] capitalize on the remarkable genome size variation in maize and its wild relatives, which differ by 40%–70% within and between subspecies. It is estimated that 85% of the maize genome is composed of TEs, B chromosomes, and heterochromatic knobs subject to meiotic drive [7,8], highlighting the success of selfish genetic elements in this lineage. Nevertheless, clines of genome size along with phenotypic and environmental variables in Zea mays spp. have been well described, with a number of studies showing evidence of genome reductions, including the loss of knobs and B chromosomes, in regions of high altitude and latitude [9–13]. While these parallel clines are suggestive of an adaptive process, it has been difficult to definitively demonstrate this and fully reject a role for neutral population history.

Bilinski et al. [6] add to this rich literature by using a quantitative genetics framework to conduct a test of local adaptation, recognizing that genome size is a trait governed by an immense number of small-effect loci. Moreover, it can be thought of as a quantitative trait under complete genetic control, since the variation in genome size is expected to be a simple function of the net number of insertion and deletion alleles individuals have across the genome. Under this framework, a neutral model predicts that genome size differences across a geographic region are a function of the relatedness and population structure [14] and thus determined by the extent of correlated allele frequencies between a given pair of individuals. It follows that if genome size is subject to selection, it should be more strongly correlated with altitude than expected from relatedness alone. The authors use low-coverage whole genome sequence data from three altitudinal clines to obtain detailed information not only about the kinship and structure of their samples but also about the contribution of each type of repeat to overall genome size.

Using this approach to test for local adaptation, the authors are able to reject the predictions of the neutral model, concluding that genome size differences along altitudinal clines are too extreme to be explained solely by drift (Fig 1). When considering individual repeat types, both TEs and knob repeat abundance are significantly correlated with altitude. They also find that one type of knob repeat, known as TR1, shows the strongest over-differentiation, and the signal of altitudinal adaptation on TR1 abundance remains significant even when controlling for genome size. With megabase-long heterochromatic knobs accounting for up to 10% of maize genome size variation [7,15], knobs seem to be acting as large-effect loci for genome size but, in some cases, may also be under additional selection pressures independent of their effects on genome size. Although TEs do not show signals of adaptation after controlling for genome size, the strong correlation of TE copy number with genome size and altitude implies that environmental adaptation could also be an important determinant of TE abundance mediated through its effects on genome size.

[Figure omitted. See PDF.]

Fig 1. Altitudinal clines in maize and teosinte.

Clines in heterochromatic knobs and genome size in Zea mays spp. are significantly over-dispersed compared to genome-wide allele frequencies, corresponding with an adaptive response to selection for earlier flowering time at higher altitudes. TE content is also a major determinant of genome size variation and thus also covaries significantly with altitude. TE, transposable element.

https://doi.org/10.1371/journal.pgen.1007249.g001

With the finding that natural selection shapes genome size variation across multiple parallel altitudinal clines in maize and its wild relatives, Bilinski et al. [6] searched for the phenotypic target of selection. It has been proposed that genome size evolution may be mediated by its effects on flowering time [10,13,16], given that populations at higher latitudes are typically early-flowering [17,18], and a previous selection experiment on flowering time led to a correlated decrease in genome size [16]. This could result from the strong scaling between genome size, cell size, and cell cycle length [19]. Bilinski et al. [6] were able to uncouple a role for population history driving an indirect association between genome size and these traits through performing a growth chamber experiment on a single highly variable population and controlling for family structure. They then infer a causal relationship between genome size, cell production rate, and flowering time through a path analysis. This mechanistic connection, combined with the body of past work, provides a compelling case for flowering time as the driver of altitudinal genome downsizing with large chromosomal knobs being a major factor under selection. A parallel study in maize identified the region with the most explanatory power for genome size to map near two 180-bp knobs where genome size was also correlated with flowering time, but only before controlling for relatedness—perhaps due to greater effects of population structure in their study [20]. Although the fraction of flowering time variation controlled by genome size is still unclear, Bilinski et al. [6] add to growing evidence for the case of genome size evolution driven by phenotypic selection, in line with a recent large-scale phylogenetic study that argues genome downsizing was the key to angiosperms’ higher photosynthetic efficiency [21].

This paper provides important evidence for the organismal-adaptation view of genome size evolution—but what of the coevolutionary struggle with selfish elements? The major evolutionary force driving genome expansion in maize is still likely to reflect selfish evolution through selection on meiotic drivers and self-replicating TEs, since selection pressures on such alleles are typically very strong. Knob elements, for example, have a strong transmission advantage driving their increase [22]. On the other hand, the factors stabilizing unregulated genome expansion or driving genome shrinkage may be multifaceted. The explosion of selfish genetic elements is typically thought to be controlled by unconditionally deleterious effects such as meiotic defects on pollen viability and seed set [22], ectopic recombination events causing chromosomal rearrangements [23–25], and the harmful effects of gene disruption. However, the results found by Bilinski et al. [6] highlight that some costs to selfish genetic elements may be environmentally dependent, determined by the indirect effects of repetitive elements on flowering time through their effects on genome size. In regions of high elevation, stronger selection against large genomes could lead to greater control of total repetitive element abundance. For large-effect alleles such as chromosomal knobs, such environmentally dependent selection could explain why their population frequencies are somewhat lower than expected by their effects on pollen viability and seed set alone [22].

Conversely, other selective pressures on selfish genetic elements may provide an additional source of genome size variation in maize populations beyond the effects of flowering time. Since chromosomal knobs are known to have severe fitness costs, the extent of these costs may well depend on the environment [22], possibly explaining the disproportionate effect of altitude on knobs when genome size is controlled for in this study. For instance, if conditions are more extreme at higher elevations, the costs of knobs on fecundity may be felt more severely in the absence of buffering from favorable conditions. The costs of TEs and heterochromatic knobs may also be more severe in inbred genomic backgrounds for example; since knobs have recessive deleterious costs [22], they should be subject to strong purifying selection when homozygous. Consistent with this, inbred lines of domesticated maize have been found to be mostly absent of knobs [26]. This may explain why the smallest genomes in the present study were found in the three lowest-altitude teosinte populations, providing an exception to the observed altitudinal gradient. These populations are known to be highly inbred, although admixture from related species may also be playing a role. Genome size variation in maize may thus result from the dynamic interplay between the selfish spread of knobs and TEs, heterogeneity in their costs and the efficacy of selection against them, and the exaptation of the resulting variation for the optimization of developmental traits such as flowering time. This suggests a pluralistic model whereby selfish evolution, environmental adaptation, and population history all contribute to evolutionary fluctuations in DNA content.

Acknowledgments

We are grateful to Maud Tenaillon for helpful discussion and comments on the manuscript.

Citation: Kreiner JM, Wright SI (2018) A less selfish view of genome size evolution in maize. PLoS Genet 14(5): e1007249. https://doi.org/10.1371/journal.pgen.1007249

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