Abstract

Extensive rodent literature suggests that the endocannabinoid (eCB) system present in the nucleus accumbens (NAc) modulates dopamine (DA) release in this area. However, expression patterns of the cannabinoid receptor type 1 (CB1R), the synthesizing enzyme N-acyl phosphatidylethanolamine phospholipase D (NAPE-PLD), and the degradation enzyme fatty acid amide hydrolase (FAAH) in the NAc have not yet been described in non-human primates. The goal of this study is therefore to characterize the expression and localization of the eCB system within the NAc of vervet monkeys (Chlorocebus sabaeus) using Western blots and immunohistochemistry. Results show that CB1R, NAPE-PLD, and FAAH are expressed across the NAc rostrocaudal axis, both in the core and shell. CB1R, NAPE-PLD, and FAAH are localized in medium spiny neurons (MSNs) and fast-spiking GABAergic interneurons (FSIs). Dopaminergic projections and astrocytes did not express CB1R, NAPE-PLD, or FAAH. These data show that the eCB system is present in the vervet monkey NAc and supports its role in the primate brain reward circuit.

Details

Title
Expression and localization of CB1R, NAPE-PLD, and FAAH in the vervet monkey nucleus accumbens
Author
Kucera, Ryan 1 ; Bouskila, Joseph 2 ; Elkrief, Laurent 3 ; Fink-Jensen, Anders 4 ; Palmour, Roberta 5 ; Bouchard, Jean-François 1   VIAFID ORCID Logo  ; Ptito, Maurice 6 

1  School of Optometry, University of Montreal, Montreal, QC, Canada 
2  School of Optometry, University of Montreal, Montreal, QC, Canada; Department of Psychiatry and Human Genetics, McGill University, Montreal, QC, Canada; St‐Kitts Behavioural Science Foundation, West Indies, Caribbean, Saint Kitts and Nevis 
3  School of Optometry, University of Montreal, Montreal, QC, Canada; Faculty of Medicine, University of Montreal, Montreal, QC, Canada 
4  Laboratory of Neuropsychiatry, Psychiatric Centre Copenhagen, Copenhagen, Denmark 
5  Department of Psychiatry and Human Genetics, McGill University, Montreal, QC, Canada; St‐Kitts Behavioural Science Foundation, West Indies, Caribbean, Saint Kitts and Nevis 
6  School of Optometry, University of Montreal, Montreal, QC, Canada; St‐Kitts Behavioural Science Foundation, West Indies, Caribbean, Saint Kitts and Nevis; Laboratory of Neuropsychiatry, Psychiatric Centre Copenhagen, Copenhagen, Denmark 
Pages
1-12
Publication year
2018
Publication date
Jun 2018
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-018-26826-2
ProQuest document ID
2051032460
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.