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Copyright © 2014 Francisca Valéria Soares de Araújo Pinho et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0/

Abstract

Duguetia furfuracea is frequently used as a medicinal plant in Brazil. However, studies have evidenced its cytotoxic, bactericide, and antitumor activities. In the present study we aimed to evaluate the potential toxicity of hydroalcoholic leaves extracts of D. furfuracea (HEDF) in a Drosophila melanogaster model. Toxicity was assessed as changes in locomotor performance, mitochondrial activity, oxidative stress, MAPKs phosphorylation, and apoptosis induction after exposure to HEDF concentrations (1–50 mg/mL) for 7 days. The phytoconstituents of the plant were screened for the presence of alkaloids, tannins, xanthones, chalcones, flavonoids, aurones, and phenolic acids. Exposure of adult flies to HEDF caused mitochondrial dysfunction, overproduction of ROS, and alterations in the activity of detoxifying enzymes GST, SOD and CAT. Induction of ERK phosphorylation and PARP cleavage was also observed, indicating occurrence of HEDF-induced cell stress and apoptotic cell death. In parallel, alterations in cholinesterase activity and impairments in negative geotaxis behavior were observed. Our study draws attention to the indiscriminate use of this plant by population and suggests oxidative stress as a major mechanism underlying its toxicity.

Details

Title
Phytochemical Constituents and Toxicity of Duguetia furfuracea Hydroalcoholic Extract in Drosophila melanogaster
Author
Francisca Valéria Soares de Araújo Pinho 1 ; Gustavo Felipe da Silva 2 ; Giulianna Echeverria Macedo 2 ; Muller, Katiane Raquel 2 ; Martins, Illana Kemmerich 2 ; Ana Paula Lausmann Ternes 2   VIAFID ORCID Logo  ; José Galberto Martins da Costa 1 ; Margareth Linde Athayde 3 ; Aline Augusti Boligon 3 ; Kamdem, Jean Paul 4 ; Franco, Jeferson Luis 2   VIAFID ORCID Logo  ; Irwin Rose Alencar de Menezes 1 ; Posser, Thaís 2   VIAFID ORCID Logo 

 Departamento de Química Biológica, Universidade Regional do Cariri, 63100-000 Crato, CE, Brazil 
 Centro Interdisciplinar de Pesquisa em Biotecnologia, Universidade Federal do Pampa, Campus São Gabriel, 97300-000 São Gabriel, RS, Brazil 
 Departamento de Farmácia Industrial, Laboratório de Pesquisa em Fitoquímica, Universidade Federal de Santa Maria, Prédio 26, Sala 1115, 97105-900 Santa Maria, RS, Brazil 
 Departamento de Química, Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil 
Editor
Wenchuan Lin
Publication year
2014
Publication date
2014
Publisher
John Wiley & Sons, Inc.
ISSN
1741427X
e-ISSN
17414288
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2062803039
Copyright
Copyright © 2014 Francisca Valéria Soares de Araújo Pinho et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0/