Reconstructing complex lineage trees from scRNA-seq data using MERLoT

Abstract

Advances in single-cell transcriptomics techniques are revolutionising studies of cellular differentiation and heterogeneity. However, reconstruction of cellular lineage trees with more than a few cell fates has proved challenging. We present MERLoT (https://github.com/soedinglab/merlot), a tool to reconstruct complex lineage trees from single-cell transcriptomics data. We demonstrate MERLoT's robustness, flexibility and capabilities on various real and simulated datasets.

Footnotes

* Benchmark was updated. Code was updated to be faster. A section was added where we show the reconstruction of a gene regulatory network using imputed gene expression profiles.