Gene expression pattern of TCR repertoire and alteration expression of IL-17A gene of γδ T cells in patients with acute myocardial infarction

Abstract

Table 2 Baseline demographic data of enrolled subjects AMI Healthy controls P value Number 25 14 NA Age (years) (median ± range) 64.4 ± 11.7 57.3 ± 9.5 0.060 Gender (male) 17 (68%) 8 (57%) NA Arterial hypertension 18 (72%) 0 NA Diabetes mellitus 6 (24%) 0 NA Prior myocardial infarction 0 0 NA Dyslipoproteinaemia 11 (44%) 7 (50%) NA History of smoking 9 (36%) 0 NA WBC (× 109/L) 10.85 ± 3.45 6.97 ± 2.17 0.001 Total cholesterol (mmol/L) 5.09 ± 0.97 5.23 ± 0.85 0.672 LDL-C (mmol/L) 3.16 ± 0.80 3.11 ± 0.64 0.884 HDL-C (mmol/L) 1.12 ± 2.56 1.52 ± 0.32 0.002 AMI acute myocardial infarction, WBC white blood cells, LDL-C low-density lipoprotein cholesterol, HDL-C high-density lipoprotein cholesterol, hs-CRP high-sensitivity C-reactive protein Table 3 Biochemical and clinical data of the AMI patients AMI Number 25 hs-CRP (mg/L) 11.13 ± 11.65 Cardiac troponin I (pg/mL) 24.78 ± 15.06 NT-proBNP (ng/mL) 1266.73 ± 1685.79 LVEF (%) 51 ± 9 Infarct-related artery (no.) LAD 24 RCA 4 LCX 6 LM 1 Killip at admission Killip 1 7 Killip ≥ 2 18 NT-proBNP N-terminal pro B-type natriuretic peptide, LVEF left ventricular ejected fraction, LAD left anterior descending branch coronary artery, LCX left circumflex artery, LM left main coronary artery, RCA right coronary artery Expression pattern and clonality of TCR γδ T cells in AMI patients Quantitative analysis of mRNA expression levels of TCR Vγ subfamilies genes in γδ T cells of AMI patients and healthy individuals showed that the expression of TCR Vγ 1–3 genes were higher in AMI patients compared with that in healthy controls (0.43 ± 0.41% vs. 0.06 ± 0.09%, P = 0.0003 for Vγ1; 0.35 ± 0.42% vs. 0.03 ± 0.03%, P = 0.001 for Vγ2; 0.25 ± 0.29% vs. 0.03 ± 0.05%, P = 0.001 for Vγ3) (Fig. 1). According to the different peptide chains, the human peripheral blood T cells are divided into two subgroup populations-αβ T cells and γδ T cells. Data of animal experiments indicate that the number of naturally occurring CD4+CD25+ Tregs is associated with autoimmune diseases as well as atherosclerosis. [...]evidence showed that Foxp3 is the most specific marker for monitoring the development and function of CD4+CD25+ Tregs. Even though the immunologic function of γδ T cells in AMI is unknown, an important role of γδ T cells can be speculated. [...]these findings may well reveal novel therapeutic options for those who suffered from AMI.