Molecular characterization of a putative serine protease from Trichinella spiralis and its elicited immune protection

Abstract

There are increasing cosmopolitan demands for reliable preventive measures for Trichinella infection in food animals to ensure meat safety [9]. [...]the exploitation of vaccines to prevent domestic swine from Trichinella infection is a promising measure for the control of this zoonosis [10–13]. [...]the slides were treated with 70% glycerol and observed with a fluorescent microscope (Olympus, Japan). Upon completion of the staining procedure, the coverslips were transferred to glass slides and observed with fluorescent microscopy (Olympus, Tokyo, Japan) [33]. [...]cellular localization of rTsSP within enterocytes was further examined with confocal microscopy and analyzed with Olympus Fluoview software [30]. Immune protective effects of rTsSP The mice immunized with rTsSP produced a 52.70% reduction of intestinal adult worm burden at 5 dpi and a 52.10% reduction of muscle larval burden at 42 dpi (Figure 15), in comparison with the PBS control group (Fadult = 55.914, Flarva = 12.612, P < 0.01). [...]the adult and larval burden of immunized mice was also significantly lower than those of the adjuvant-injected group alone (P < 0.01).