Abstract

Owing to the recent progress in regenerative medicine technology, clinical trials that harnessed the regeneration and immune modulation potentiality of stem cells for treating IBD have shown promising results. We investigated the feasibility and utility of intraluminal endoscopic transplantation of rat MSC sheets in murine models of experimental colitis for targeted delivery of stem cells to lesions. We isolated adipose-derived mesenchymal stem cells (AD-MSC) and bone marrow-derived mesenchymal stem cells (BM-MSC) from EGFP-transgenic rats and fabricated the cells in sheet forms using temperature-responsive culture dishes. The MSC sheets were endoscopically transplanted to the inflamed area in electrocoagulation and DNBS colitis model. The effect of the transplantation was verified using endoscopic scoring and histological analysis. In the electrocoagulation model, the AD-MSC group showed significantly decreased ulcer size in the transplanted regions. In the DNBS colitis model, the AD-MSC group showed decreased inflammation and colitis in the transplanted regions. Histologic analysis showed that the MSC sheets had successfully attached to the inflamed mucosa in both the electrocoagulation and DNBS colitis model. Our results show that endoscopic transplantation of MSC sheets could be a new effective mode of stem cell therapy for IBD treatment.

Details

Title
Endoscopic Transplantation of Mesenchymal Stem Cell Sheets in Experimental Colitis in Rats
Author
Pak, Sehyung 1 ; Hwang, Sung Wook 2 ; In Kyong Shim 1   VIAFID ORCID Logo  ; Bae, Sang Mun 1 ; Ryu, Yeon- Mi 1 ; Han-Byul, Kim 1 ; Eun-ju Do 1 ; Hye-Nam Son 1 ; Eun-ji, Choi 1 ; Sun-ha, Park 1 ; Kim, Sang-Yeob 1 ; Park, Sang Hyoung 2 ; Byong Duk Ye 2   VIAFID ORCID Logo  ; Suk-Kyun Yang 2 ; Kanai, Nobuo 3 ; Maeda, Masanori 4 ; Okano, Teruo 5 ; Dong-Hoon, Yang 2 ; Jeong-Sik Byeon 2 ; Seung-Jae Myung 6 

1  Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea 
2  Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea 
3  Research center, Tokyo Metropolitan Geriatric Medical Center, Tokyo, Japan 
4  Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo, Japan 
5  Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo, Japan; Cell Sheet Tissue Engineering Center, Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, Utah, USA 
6  Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea; Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea 
Pages
1-11
Publication year
2018
Publication date
Jul 2018
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-018-29617-x
ProQuest document ID
2077457226
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.