Abstract

In this study, we used an argon-based round atmospheric-pressure plasma jet (APPJ) for enhancing wound healing in streptozotocin (STZ) induced diabetic rats. The APPJ was characterized by optical emission spectroscopy. We induced Type 1 and Type 2 diabetes in rats with different amounts of STZ combined with normal and high-fat diets, respectively. The wound area ratio of all the plasma-treated normal and diabetic groups was greatly reduced (up to 30%) compared with that of the untreated groups during healing. Histological analysis revealed faster re-epithelialization, collagen deposition, less inflammation, and a complete skin structure in the plasma-treated groups was found as compared with the untreated control groups. In addition, the new blood vessels of plasma-treated tissues decreased more than untreated tissues in the middle (Day 14) and late (Day 21) stages of wound healing. The plasma-treated wounds demonstrated more transforming growth factor beta (TGF-β) expression in the early stage (Day 7), whereas they decreased in the middle and late stages of wound healing. The levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) increased after plasma treatment. In addition, plasma-treated water had a higher concentration of hydrogen peroxide, nitrite and nitrate when the plasma treatment time was longer. In summary, the proposed argon APPJ based on the current study could be a potential tool for treating diabetic wounds.

Details

Title
Wound Healing in Streptozotocin-Induced Diabetic Rats Using Atmospheric-Pressure Argon Plasma Jet
Author
Kuang-Yao, Cheng 1 ; Zhi-Hua, Lin 1 ; Yu-Pin, Cheng 2 ; Hsien-Yi Chiu 3 ; Yeh, Nai-Lun 4 ; Tung-Kung, Wu 5   VIAFID ORCID Logo  ; Jong-Shinn, Wu 1   VIAFID ORCID Logo 

1  Department of Mechanical Engineering, National Chiao Tung University, Hsinchu, Taiwan 
2  Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan; Department of Dermatology, Cathay General Hospital, Taipei, Taiwan 
3  Department of Dermatology, National Taiwan University Hospital Hsinchu Branch, Hsinchu, Taiwan 
4  Department of Family Medicine, National Taiwan University Hospital Hsinchu Branch, Hsinchu, Taiwan 
5  Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan 
Pages
1-15
Publication year
2018
Publication date
Aug 2018
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-018-30597-1
ProQuest document ID
2088919784
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.