Abstract

The value of exotic wheat genetic resources for accelerating grain yield gains is largely unproven and unrealized. We used next-generation sequencing, together with multi-environment phenotyping, to study the contribution of exotic genomes to 984 three-way-cross-derived (exotic/elite1//elite2) pre-breeding lines (PBLs). Genomic characterization of these lines with haplotype map-based and SNP marker approaches revealed exotic specific imprints of 16.1 to 25.1%, which compares to theoretical expectation of 25%. A rare and favorable haplotype (GT) with 0.4% frequency in gene bank identified on chromosome 6D minimized grain yield (GY) loss under heat stress without GY penalty under irrigated conditions. More specifically, the ‘T’ allele of the haplotype GT originated in Aegilops tauschii and was absent in all elite lines used in study. In silico analysis of the SNP showed hits with a candidate gene coding for isoflavone reductase IRL-like protein in Ae. tauschii. Rare haplotypes were also identified on chromosomes 1A, 6A and 2B effective against abiotic/biotic stresses. Results demonstrate positive contributions of exotic germplasm to PBLs derived from crosses of exotics with CIMMYT’s best elite lines. This is a major impact-oriented pre-breeding effort at CIMMYT, resulting in large-scale development of PBLs for deployment in breeding programs addressing food security under climate change scenarios.

Details

Title
Harnessing genetic potential of wheat germplasm banks through impact-oriented-prebreeding for future food and nutritional security
Author
Singh, Sukhwinder 1 ; Vikram, Prashant 1   VIAFID ORCID Logo  ; Sehgal, Deepmala 1 ; Burgueño, Juan 1 ; Sharma, Achla 2 ; Singh, Sanjay K 3 ; Sansaloni, Carolina P 1 ; Ryan Joynson 4 ; Brabbs, Thomas 4 ; Ortiz, Cynthia 1 ; Solis-Moya, Ernesto 5 ; Velu Govindan 1   VIAFID ORCID Logo  ; Gupta, Naveen 6 ; Sidhu, Harminder S 6 ; Basandrai, Ashwani K 7   VIAFID ORCID Logo  ; Basandrai, Daisy 7 ; Ledesma-Ramires, Lourdes 5 ; Suaste-Franco, Maria P 5 ; Fuentes-Dávila, Guillermo 8 ; Moreno, Javier I 9 ; Sonder, Kai 1   VIAFID ORCID Logo  ; Singh, Vaibhav K 10 ; Singh, Sanjay 11 ; Shokat, Sajid 12 ; Arif, Mian A R 13 ; Laghari, Khalil A 14 ; Srivastava, Puja 2 ; Sridhar Bhavani 15 ; Kumar, Satish 3 ; Pal, Dharam 16 ; Jaiswal, Jai P 17 ; Kumar, Uttam 6 ; Chaudhary, Harinder K 7 ; Crossa, Jose 1   VIAFID ORCID Logo  ; Payne, Thomas S 1 ; Muhammad Imtiaz 18 ; Sohu, Virinder S 2 ; Singh, Gyanendra P 3 ; Bains, Navtej S 2 ; Hall, Anthony 19 ; Pixley, Kevin V 1 

 International Maize and Wheat Improvement Center (CIMMYT), Texcoco, C.P., Mexico 
 Department Plant Breeding & Genetics, Punjab Agriculture University, Ludhiana, India 
 ICAR-Indian Institute of Wheat and Barley Research, Karnal, India 
 Earlham Institute, Norwich, Norfolk, UK 
 Carretera Celaya-San Miguel de Allende, Celaya, Guanajuato, Mexico 
 Borlaug Institute for South Asia (BISA), CIMMYT, Ladhowal, Punjab, India 
 CSK Himachal Pradesh Agricultural University Palampur, Palampur, Himachal Pradesh, India 
 INIFAP-CIRNO, Campo Experimental Norman E. Borlaug, Sonora, C.P., Mexico 
 INIFAP, Interior Parque Los Colomos S/N, Colonia Providencia, CP, Guadalajara, Jalisco, Mexico 
10  ICAR-Indian Agricultural Research Institute (IARI), New Delhi, India 
11  National Research Center for Plant Biotechnology, New Delhi, India 
12  Nuclear Institute for Agriculture and Biology, Faislabad, Pakistan; Department of Plant and Environmental Sciences, Crop Science, University of Copenhagen, Taastrup, Denmark 
13  Nuclear Institute for Agriculture and Biology, Faislabad, Pakistan 
14  Nuclear Institute of Agriculture, Tando Jam, Sindh, Pakistan 
15  CIMMYT - World Agroforestry Centre (ICRAF), United Nations Avenue, Nairobi, Kenya 
16  ICAR-Indian Agricultural Research Institute, Regional Station, Shimla, India 
17  Department of Genetics and Plant Breeding, G.B. Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, India 
18  CIMMYT – Pakistan, NARC CSI Complex, Park Road, Islamabad, Pakistan 
19  Earlham Institute, Norwich, Norfolk, UK; School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, UK 
Pages
1-11
Publication year
2018
Publication date
Aug 2018
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2091208361
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.