Abstract

Tumor aggressiveness is usually associated with metastasis. MDA-MB 231, a triple-negative breast cancer (TNBC), is an aggressive type of breast cancer and associated with early metastasis. The Rho/ROCK pathway is a key regulator of cell motility involving cytoskeleton regulation through stabilization of actin filaments and stress fiber formation. In this study we show that Fasudil, a ROCK inhibitor, inhibited the migration of MDA-MB 231 and A549 cells, without altering the viability of these cells at the concentration of 10 μM, modified tumor cell morphology, with disorganization of stress fibers and promotes activation of the canonical-Wnt/beta-catenin pathway. Therefore, Fasudil present a promising approach to the prevention of breast cancer metastasis through a different mechanism of action from the well-known one.

Details

Title
ROCK inhibition with Fasudil induces beta-catenin nuclear translocation and inhibits cell migration of MDA-MB 231 human breast cancer cells
Author
Guerra, Fabiana Sélos 1 ; Guerra de Oliveira, Ramon 2 ; Carlos Alberto Manssour Fraga 2 ; Claudia dos Santos Mermelstein 3 ; Patricia Dias Fernandes 1   VIAFID ORCID Logo 

1  Universidade Federal do Rio de Janeiro, Instituto de Ciências Biomédicas, Laboratório de Farmacologia da Dor e da Inflamação, Rio de Janeiro, Brazil 
2  Universidade Federal do Rio de Janeiro, Instituto de Ciências Biomédicas, Laboratório de Avaliação e Síntese de Substâncias Bioativas (LASSBio®), Rio de Janeiro, Brazil 
3  Universidade Federal do Rio de Janeiro, Instituto de Ciências Biomédicas, Laboratório de Diferenciação Muscular, Rio de Janeiro, Brazil 
Pages
1-8
Publication year
2017
Publication date
Oct 2017
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-017-14216-z
ProQuest document ID
2123042596
Copyright
© 2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.