Abstract

Control of translation is vital to all species. Here we employ a multi-omics approach to decipher condition-dependent translational regulation in the model acetogen Clostridium ljungdahlii. Integration of data from cells grown autotrophically or heterotrophically revealed that pathways critical to carbon and energy metabolism are under strong translational regulation. Major pathways involved in carbon and energy metabolism are not only differentially transcribed and translated, but their translational efficiencies are differentially elevated in response to resource availability under different growth conditions. We show that translational efficiency is not static and that it changes dynamically in response to mRNA expression levels. mRNAs harboring optimized 5′-untranslated region and coding region features, have higher translational efficiencies and are significantly enriched in genes encoding carbon and energy metabolism. In contrast, mRNAs enriched in housekeeping functions harbor sub-optimal features and have lower translational efficiencies. We propose that regulation of translational efficiency is crucial for effectively controlling resource allocation in energy-deprived microorganisms.

Details

Title
Optimization of carbon and energy utilization through differential translational efficiency
Author
Al-Bassam, Mahmoud M 1 ; Ji-Nu, Kim 1 ; Zaramela, Livia S 1 ; Kellman, Benjamin P 2   VIAFID ORCID Logo  ; Zuniga, Cristal 1 ; Wozniak, Jacob M 3 ; Gonzalez, David J 3 ; Zengler, Karsten 4   VIAFID ORCID Logo 

1  Department of Pediatrics, Division of Host−Microbe Systems and Therapeutics, University of California San Diego, La Jolla, CA, USA 
2  Department of Pediatrics, Division of Host−Microbe Systems and Therapeutics, University of California San Diego, La Jolla, CA, USA; Bioinformatics and Systems Biology Program, University of California, La Jolla, CA, USA 
3  Department of Pharmacology, University of California San Diego, La Jolla, CA, USA; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA 
4  Department of Pediatrics, Division of Host−Microbe Systems and Therapeutics, University of California San Diego, La Jolla, CA, USA; Center for Microbiome Innovation, University of California San Diego, La Jolla, CA, USA 
Pages
1-13
Publication year
2018
Publication date
Oct 2018
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-018-06993-6
ProQuest document ID
2125648131
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.