Correlation of microsatellite alterations on chromosome 9 and oncogenesis, metastasis in NSCLC

Abstract

目的 探讨人类第9号染色体短臂(9p)上微卫星改变与肺癌发生及转移的相关性。方法 应用聚合酶链反应(PCR)结合微卫星银染分析方法检测32例非小细胞肺癌(NSCLC)组织中两个微卫星标志物IFNA和D9S171的改变。结果 32例NSCLC患者中，14例(43.8％)出现了微卫星标志物改变。其中伴有淋巴结转移的肺癌组织中微卫星改变检出率为64.7％，不伴转移的肺癌组织中微卫星改变检出率为20％，两组间比较有显著性差异(P＜0.05)。16例良性肺部疾病组织均未检出微卫星改变。结论 人类第9号染色体微卫星改变在NSCLC中普遍存在，有可能成为肺癌早期诊断和转移判断的分子标志物。

Objective To investigate the relationship among microsatellite alterations on chromosome 9(9p) and the oncogenesis,metastasis of non-small cell lung cancer (NSCLC).Methods Two microsatellite markers,IFNA and D9S171,were analyzed in 32 cases of NSCLC by polymerase chain reaction-based silver-staining assay.Results Of 32 cases of NSCLC,14(43.8％) had microsatellite alterations.The alteration frequency was 64.7％ in NSCLC with lymph node metastasis,and 20％ in those without lymph node metastasis.There was significant difference between the two groups.None of benign lung tissues showed microsatellite alterations.Conclusion Microsatellite alteration on chromosome 9p in NSCLC appears to be a common and specific event,and may be a valuable molecular marker for determination of early diagnosis and metastasis of NSCLC.