Abstract

The near infrared (NIR) optical window between the cutoff for hemoglobin absorption at 650 nm and the onset of increased water absorption at 900 nm is an attractive, yet largely unexplored, spectral regime for diffraction-unlimited super-resolution fluorescence microscopy (nanoscopy). We developed the NIR fluorescent protein SNIFP, a bright and photostable bacteriophytochrome, and demonstrate its use as a fusion tag in live-cell microscopy and STED nanoscopy. We further demonstrate dual color red-confocal/NIR-STED imaging by co-expressing SNIFP with a conventional red fluorescent protein.

Details

Title
Near-infrared STED nanoscopy with an engineered bacterial phytochrome
Author
Kamper, Maria 1 ; Ta, Haisen 1 ; Jensen, Nickels A 1 ; Hell, Stefan W 1 ; Jakobs, Stefan 2   VIAFID ORCID Logo 

1  Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany 
2  Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany; Department of Neurology, University Medical Center Göttingen, Göttingen, Germany 
Pages
1-7
Publication year
2018
Publication date
Nov 2018
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-018-07246-2
ProQuest document ID
2132241359
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.