Abstract

Pectin-rich biomasses, such as citrus peel and sugar beet pulp, hold promise as inexpensive feedstocks for microbial fermentations as enzymatic hydrolysis of their component polysaccharides can be accomplished inexpensively to yield high concentrations of fermentable sugars and d-galacturonic acid (d-galUA). In this study, we tackle a number of challenges associated with engineering a microbial strain to convert pectin-rich hydrolysates into commodity and specialty chemicals. First, we engineer d-galUA utilization into yeast, Saccharomyces cerevisiae. Second, we identify that the mechanism of d-galUA uptake into yeast is mediated by hexose transporters and that consumption of d-galUA is inhibited by d-glucose. Third, we enable co-utilization of d-galUA and d-glucose by identifying and expressing a heterologous transporter, GatA, from Aspergillus niger. Last, we demonstrate the use of this transporter for production of the platform chemical, meso-galactaric acid, directly from industrial Navel orange peel waste.

Details

Title
Engineering Saccharomyces cerevisiae for co-utilization of d-galacturonic acid and d-glucose from citrus peel waste
Author
Protzko, Ryan J 1 ; Latimer, Luke N 2 ; Martinho, Ze 3 ; de Reus, Elise 4 ; Seibert, Tanja 5 ; Benz, J Philipp 6   VIAFID ORCID Logo  ; Dueber, John E 7   VIAFID ORCID Logo 

1  Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA 
2  Department of Chemistry, University of California, Berkeley, CA, USA 
3  Department of Bioengineering, University of California, Berkeley, CA, USA 
4  Department of Biotechnology and Biomedicine, Technical University of Denmark, Kgs Lyngby, Denmark 
5  Holzforschung München, TUM School of Life Sciences Weihenstephan, Technische Universität München, Freising, Germany; Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany 
6  Holzforschung München, TUM School of Life Sciences Weihenstephan, Technische Universität München, Freising, Germany 
7  Department of Bioengineering, University of California, Berkeley, CA, USA; Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA 
Pages
1-10
Publication year
2018
Publication date
Nov 2018
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-018-07589-w
ProQuest document ID
2139592268
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.