Abstract

Pathogen co-evolution with plants involves selection for evasion of host surveillance systems. The oomycete Hyaloperonospora arabidopsidis (Hpa) causes downy mildew on Arabidopsis, and race-specific interactions between Arabidopsis accessions and Hpa isolates fit the gene-for-gene model in which host resistance or susceptibility are determined by matching pairs of plant Resistance (R) genes and pathogen Avirulence (AVR) genes. Arabidopsis Col-0 carries R gene RPP4 that confers resistance to Hpa isolates Emoy2 and Emwa1, but its cognate recognized effector(s) were unknown. We report here the identification of the Emoy2 AVR effector gene recognized by RPP4 and show resistance-breaking isolates of Hpa on RPP4-containing Arabidopsis carry the alleles that either are not expressed, or show cytoplasmic instead of nuclear subcellular localization.

Details

Title
A downy mildew effector evades recognition by polymorphism of expression and subcellular localization
Author
Asai, Shuta 1 ; Furzer, Oliver J 2 ; Cevik, Volkan 3 ; Dae Sung Kim 4 ; Ishaque, Naveed 5 ; Goritschnig, Sandra 6 ; Staskawicz, Brian J 6 ; Shirasu, Ken 7   VIAFID ORCID Logo  ; Jones, Jonathan D G 8 

1  Center for Sustainable Resource Science, RIKEN, Yokohama, Kanagawa, Japan; The Sainsbury Laboratory, Norwich, UK 
2  The Sainsbury Laboratory, Norwich, UK; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA 
3  The Sainsbury Laboratory, Norwich, UK; Department of Biology & Biochemistry, University of Bath, Bath, UK 
4  The Sainsbury Laboratory, Norwich, UK; Department of Plant Sciences, College of Life Sciences, Wuhan University, Wuhan, China 
5  The Sainsbury Laboratory, Norwich, UK; Heidelberg Center for Personalized Oncology, DKFZ-HIPO, DKFZ, Heidelberg, Germany 
6  Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA 
7  Center for Sustainable Resource Science, RIKEN, Yokohama, Kanagawa, Japan 
8  The Sainsbury Laboratory, Norwich, UK 
Pages
1-11
Publication year
2018
Publication date
Dec 2018
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-018-07469-3
ProQuest document ID
2150519995
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.