Abstract

Peptidoglycan-binding protein-modified magnetic nanobeads (PGBP-MNBs) were prepared for efficient magnetic capturing of Staphylococcus aureus (S. aureus), which is associated with sepsis, using the binding affinity of PGBP for the peptidoglycan (PG) layer on S. aureus. These PGBP-MNBs can simply capture S. aureus in plasma within 1 hr or even 15 min. Importantly, they also can capture various types of Gram-positive bacteria, such as Bacillus cereus and methicillin-resistant and methicillin-susceptible S. aureus (MRSA and MSSA). We believe that PGBP-based systems will be used to develop diagnostic systems for Gram-positive bacteria-related diseases.

Details

Title
Peptidoglycan binding protein (PGBP)-modified magnetic nanobeads for efficient magnetic capturing of Staphylococcus aureus associated with sepsis in blood
Author
Lim Jaewoo 1 ; Choi, Jongmin 2 ; Guk Kyeonghye 1 ; Son Seong Uk 3 ; Lee Do Kyung 2 ; Soo-Jin, Yeom 4 ; Kang Taejoon 1   VIAFID ORCID Logo  ; Jung Juyeon 1 ; Lim, Eun-Kyung 1 

1  Korea Research Institute of Bioscience and Biotechnology (KRIBB), Hazards Monitoring Bionano Research Center, Yuseong-gu, South Korea (GRID:grid.249967.7) (ISNI:0000 0004 0636 3099) ; KRIBB School of Biotechnology, University of Science and Technology (UST), Department of Nanobiotechnology, Yuseong-gu, Republic of Korea (GRID:grid.412786.e) (ISNI:0000 0004 1791 8264) 
2  BioNano Health Guard Research Center, Yuseong-gu, South Korea (GRID:grid.249967.7) (ISNI:0000 0004 0636 3099) 
3  Korea Research Institute of Bioscience and Biotechnology (KRIBB), Hazards Monitoring Bionano Research Center, Yuseong-gu, South Korea (GRID:grid.249967.7) (ISNI:0000 0004 0636 3099) 
4  Korea Research Institute of Bioscience and Biotechnology (KRIBB), Synthetic Biology & Bioengineering Research Center, Yuseong-gu, South Korea (GRID:grid.249967.7) (ISNI:0000 0004 0636 3099) 
Publication year
2019
Publication date
Jan 2019
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-018-37194-2
ProQuest document ID
2167308301
Copyright
This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.