Abstract

The advent of nanomedicine requires novel delivery vehicles to actively target their site of action. Here, we demonstrate the development of lung-targeting drug-loaded liposomes and their efficacy, specificity and safety. Our study focuses on glucocorticoids methylprednisolone (MPS), a commonly used drug to treat lung injuries. The steroidal molecule was loaded into functionalized nano-sterically stabilized unilamellar liposomes (NSSLs). Targeting functionality was performed through conjugation of surfactant protein A (SPANb) nanobodies to form MPS–NSSLs–SPANb. MPS–NSSLs–SPANb exhibited good size distribution, morphology, and encapsulation efficiency. Animal experiments demonstrated the high specificity of MPS–NSSLs–SPANb to the lung. Treatment with MPS–NSSLs–SPANb reduced the levels of TNF-α, IL-8, and TGF-β1 in rat bronchoalveolar lavage fluid and the expression of NK-κB in the lung tissues, thereby alleviating lung injuries and increasing rat survival. The nanobody functionalized nanoparticles demonstrate superior performance to treat lung injury when compared to that of antibody functionalized systems.

Details

Title
Surfactant protein-A nanobody-conjugated liposomes loaded with methylprednisolone increase lung-targeting specificity and therapeutic effect for acute lung injury
Author
Li, Nan 1 ; Weng, Dong 2 ; Shan-Mei, Wang 2 ; Zhang, Yuan 2 ; Shan-Shan, Chen 3 ; Zhao-Fang, Yin 3 ; Zhai, Jiali 4 ; Scoble, Judy 5 ; Williams, Charlotte C 5 ; Chen, Tao 2 ; Qiu, Hui 2 ; Wu, Qin 2 ; Meng-Meng, Zhao 2 ; Li-Qin, Lu 2 ; Mulet, Xavier 4 ; Hui-Ping, Li 2 

 Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China;; Department of Respiratory Medicine, People’s Hospital Affiliated to ZhengZhou University, ZhengZhou, China; 
 Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China; 
 Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China;; School of Medicine, Suzhou University, SuZhou, China; 
 CSIRO Manufacturing, Clayton, Australia; 
 CSIRO Manufacturing, Parkville, Australia 
Pages
1770-1781
Publication year
2017
Publication date
Nov 2017
Publisher
Taylor & Francis Ltd.
ISSN
10717544
e-ISSN
15210464
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2195229249
Copyright
© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.