Full text

Turn on search term navigation

© 2015. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Oleanolic acid (OA), a naturally occurring triterpenoid, exhibits potential antitumor activity in many tumor cell lines. Gallbladder carcinoma is the most common malignancy of the biliary tract, and is a highly aggressive tumor with an extremely poor prognosis. Unfortunately, the effects of OA on gallbladder carcinoma are unknown. In this study, we investigated the effects of OA on gallbladder cancer cells and the underlying mechanism. The results showed that OA inhibits proliferation of gallbladder cancer cells in a dose-dependent and time-dependent manner on MTT and colony formation assay. A flow cytometry assay revealed apoptosis and G0/G1 phase arrest in GBC-SD and NOZ cells. Western blot analysis and a mitochondrial membrane potential assay demonstrated that OA functions through the mitochondrial apoptosis pathway. Moreover, this drug inhibited tumor growth in nude mice carrying subcutaneous NOZ tumor xenografts. These data suggest that OA inhibits proliferation of gallbladder cancer cells by regulating apoptosis and the cell cycle process. Thus, OA may be a promising drug for adjuvant chemotherapy in gallbladder carcinoma.

Details

Title
Oleanolic acid induces mitochondrial-dependent apoptosis and G0/G1 phase arrest in gallbladder cancer cells
Author
Huai-Feng Li; Xu-An, Wang; Shan-Shan Xiang; Yun-Ping, Hu; Jiang, Lin; Yi-Jun, Shu; Mao-Lan, Li; Xiang-Song, Wu; Zhang, Fei; Yuan-Yuan, Ye; Weng, Hao; Bao, Run-Fa; Cao, Yang; Lu, Wei; Qian Dong; Ying-Bin, Liu
Pages
3017-3030
Section
Original Research
Publication year
2015
Publication date
2015
Publisher
Taylor & Francis Ltd.
e-ISSN
1177-8881
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2226594302
Copyright
© 2015. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.