Abstract

Junctional epithelium (JE), which is derived from odontogenic epithelial cells immediately after eruption, is believed to be gradually replaced by oral gingival epithelium (OGE) over a lifetime. However, the detailed process of replacement remains unclear. The aim of the present study was to clarify the process of JE replacement by OGE cells using a green fluorescent protein (GFP)–positive tooth germ transplantation method. GFP-positive JE was partly replaced by OGE cells and completely replaced on day 200 after transplantation, whereas there was no difference in the expression of integrin β4 (Itgb4) and laminin 5 (Lama5) between JE before and after replacement by OGE cells. Next, GFP-positive JE was partially resected. On day 14 after resection, the regenerated JE consisted of GFP-negative cells and also expressed both Itgb4 and Lama5. In addition, the gene expression profile of JE derived from odontogenic epithelium before gingivectomy was partly different from that of JE derived from OGE after gingivectomy. These results suggest that JE derived from the odontogenic epithelium is gradually replaced by OGE cells over time and JE derived from the odontogenic epithelium might have specific characteristics different to those of JE derived from OGE.

Details

Title
Visualization of junctional epithelial cell replacement by oral gingival epithelial cells over a life time and after gingivectomy
Author
Kato Mayu 1 ; Tanaka, Junichi 2   VIAFID ORCID Logo  ; Aizawa Ryo 3 ; Yajima-Himuro Sara 3 ; Seki Tatsuaki 3 ; Tanaka, Keisuke 1 ; Yamada Atsushi 4 ; Ogawa Miho 5 ; Kamijo Ryutaro 4 ; Tsuji Takashi 5 ; Mishima Kenji 2   VIAFID ORCID Logo  ; Yamamoto Matsuo 3 

1  Showa University, Department of Periodontology, School of Dentistry, Tokyo, Japan (GRID:grid.410714.7) (ISNI:0000 0000 8864 3422); Showa University, Division of Pathology, Department of Oral Diagnostic Sciences, School of Dentistry, Tokyo, Japan (GRID:grid.410714.7) (ISNI:0000 0000 8864 3422) 
2  Showa University, Division of Pathology, Department of Oral Diagnostic Sciences, School of Dentistry, Tokyo, Japan (GRID:grid.410714.7) (ISNI:0000 0000 8864 3422) 
3  Showa University, Department of Periodontology, School of Dentistry, Tokyo, Japan (GRID:grid.410714.7) (ISNI:0000 0000 8864 3422) 
4  Showa University, Department of Biochemistry, School of Dentistry, Tokyo, Japan (GRID:grid.410714.7) (ISNI:0000 0000 8864 3422) 
5  Laboratory for Organ Regeneration, RIKEN Center for Biosystems Dynamics Research (BDR), Kobe, Hyogo, Japan (GRID:grid.410714.7); Organ Technologies Inc., Tokyo, Japan (GRID:grid.410833.d) 
Publication year
2019
Publication date
2019
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-019-44065-x
ProQuest document ID
2228646207
Copyright
© The Author(s) 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.