Abstract

Cell adhesion mediated by selectins (expressed by activated endothelium, activated platelets, and leukocytes) binding to their resepective selectin ligands (expressed by cancer cells) may be involved in metastasis. Therefore, methods of characterizing selectin ligands expressed on human tissue may serve as valuable assays. Presented herein is an innovative method for detecting functional selectin ligands expressed on human tissue that uses a dynamic approach, which allows for control over the force applied to the bonds between the probe and target molecules. This new method of tissue interrogation, known as dynamic biochemical tissue analysis (DBTA), involves the perfusion of molecular probe-coated microspheres over tissues. DBTA using selectin-coated probes is able to detect functional selectin ligands expressed on tissue from multiple cancer types at both primary and metastatic sites.

Details

Title
Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues
Author
Martin, Eric W 1   VIAFID ORCID Logo  ; Malgor Ramiro 2 ; Resto, Vicente A 3 ; Goetz, Douglas J 4 ; Burdick, Monica M 5   VIAFID ORCID Logo 

1  Russ College of Engineering and Technology, Biomedical Engineering Program, Athens, USA; Russ College of Engineering and Technology, Department of Chemical and Biomolecular Engineering, Athens, USA 
2  Russ College of Engineering and Technology, Biomedical Engineering Program, Athens, USA; Heritage College of Osteopathic Medicine, Department of Biomedical Sciences, Athens, USA 
3  University of Texas-Medical Branch, Department of Otolaryngology, Galveston, USA (GRID:grid.176731.5) (ISNI:0000 0001 1547 9964) 
4  Russ College of Engineering and Technology, Biomedical Engineering Program, Athens, USA (GRID:grid.176731.5); Russ College of Engineering and Technology, Department of Chemical and Biomolecular Engineering, Athens, USA (GRID:grid.176731.5) 
5  Russ College of Engineering and Technology, Biomedical Engineering Program, Athens, USA (GRID:grid.176731.5); Russ College of Engineering and Technology, Department of Chemical and Biomolecular Engineering, Athens, USA (GRID:grid.176731.5); Ohio University, Edison Biotechnology Institute, Athens, USA (GRID:grid.20627.31) (ISNI:0000 0001 0668 7841) 
Publication year
2019
Publication date
2019
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-019-44838-4
ProQuest document ID
2238565805
Copyright
© The Author(s) 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.