Abstract

The Microbacterium sp. LEMMJ01 isolated from Antarctic soil does not belong to any of the nearest species identified in the RDP database. Under UV radiation (A, B and C wavebands) the survival fractions of Microbacterium sp. cells were much higher compared with wild-type E. coli K12A15. Especially remarkable for an Antarctic bacterium, an expressive resistance against high UV-B doses was observed. The increased survival of DNA repair-proficient E. coli grown overnight added of 0.1 mg/ml or 1 mg/ml of the whole pigment extract produced by Microbacterium sp. revealed that part of the resistance of Microbacterium sp. against UV-B radiation seems to be connected with photoprotection by its pigments. Scanning electron microscopy revealed that UV-A and UV-B ensued membrane alterations only in E. coli. The APCI-MS fingerprints revealed the diagnostic ions for neurosporene (m/z 580, 566, 522, 538, and 524) synergism for the first time in this bacterium by HPLC-MS/MS analysis. Carotenoids also were devoid of phototoxicity and cytotoxicity effects in mouse cells and in human keratinocytes and fibroblasts.

Details

Title
Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01
Author
Maria Cristina P P Reis-Mansur 1   VIAFID ORCID Logo  ; Cardoso-Rurr, Janine S 2   VIAFID ORCID Logo  ; Josemar V Maiworm Abreu Silva 3 ; Rodrigues de Souza, Gabriela 4 ; Verônica da Silva Cardoso 1   VIAFID ORCID Logo  ; Felipe Raposo Passos Mansoldo 1 ; Pinheiro, Yuri 5 ; Schultz, Júnia 5 ; Lopez Balottin, Luciene B 3 ; Antonio Jorge Ribeiro da Silva 4 ; Lage, Claudia 2 ; Elisabete Pereira dos Santos 6 ; Alexandre Soares Rosado 5 ; Vermelho, Alane Beatriz 1   VIAFID ORCID Logo 

1  BIOINOVAR - Biocatalysis, Bioproducts and Bioenergy, Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil 
2  LaRBio – Radiations and Biology Laboratory, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil 
3  Labio/Dimav/Inmetro – Laboratory of Tissue Bioengineering/Directorate of Metrology Applied to Life Sciences/National Institute of Metrology, Quality and Technology, Duque de Caxias, Brazil 
4  Research Institute for Natural Products, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil 
5  LEMM - Laboratory of Microbial Molecular Ecology, Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil 
6  Faculty of Pharmacy, Galenico Development Laboratory, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil 
Pages
1-14
Publication year
2019
Publication date
Jul 2019
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-019-45840-6
ProQuest document ID
2251072577
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.