Abstract

The capability to encapsulate designated live cells into a biologically and mechanically tunable polymer layer is in high demand. Here, an approach to weave functional DNA polymer cocoons has been proposed as an encapsulation method. By developing in situ DNA-oriented polymerization (isDOP), we demonstrate a localized, programmable, and biocompatible encapsulation approach to graft DNA polymers onto live cells. Further guided by two mutually aided enzymatic reactions, the grafted DNA polymers are assembled into DNA polymer cocoons at the cell surface. Therefore, the coating of bacteria, yeast, and mammalian cells has been achieved. The capabilities of this approach may offer significant opportunities to engineer cell surfaces and enable the precise manipulation of the encapsulated cells, such as encoding, handling, and sorting, for many biomedical applications.

Details

Title
Design and fabrication of flexible DNA polymer cocoons to encapsulate live cells
Author
Gao, Tao 1   VIAFID ORCID Logo  ; Chen, Tianshu 2 ; Chang, Feng 3 ; He, Xiang 4 ; Mu, Chaoli 3 ; Anzai, Jun-ichi 5 ; Li, Genxi 6 

1  Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai, P.R. China; Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, P.R. China 
2  Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai, P.R. China 
3  State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, P.R. China 
4  School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, P.R. China 
5  Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan 
6  Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai, P.R. China; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, P.R. China 
Pages
1-10
Publication year
2019
Publication date
Jul 2019
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-019-10845-2
ProQuest document ID
2251710154
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.