Abstract

Recent advances in optical clearing and light-sheet microscopy have provided unprecedented access to structural and molecular information from intact tissues. However, current light-sheet microscopes have imposed constraints on the size, shape, number of specimens, and compatibility with various clearing protocols. Here we present a multi-immersion open-top light-sheet microscope that enables simple mounting of multiple specimens processed with a variety of clearing protocols, which will facilitate wide adoption by preclinical researchers and clinical laboratories. In particular, the open-top geometry provides unsurpassed versatility to interface with a wide range of accessory technologies in the future.

Details

Title
Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues
Author
Glaser, Adam K 1 ; Reder, Nicholas P 2 ; Chen, Ye 1 ; Yin, Chengbo 1 ; Linpeng Wei 1   VIAFID ORCID Logo  ; Kang, Soyoung 1   VIAFID ORCID Logo  ; Barner, Lindsey A 1 ; Xie, Weisi 1 ; McCarty, Erin F 2 ; Mao, Chenyi 3 ; Halpern, Aaron R 3 ; Stoltzfus, Caleb R 4   VIAFID ORCID Logo  ; Daniels, Jonathan S 5 ; Gerner, Michael Y 4   VIAFID ORCID Logo  ; Nicovich, Philip R 6 ; Vaughan, Joshua C 7 ; True, Lawrence D 2 ; Liu, Jonathan T C 8   VIAFID ORCID Logo 

 Department of Mechanical Engineering, University of Washington, Seattle, WA, USA 
 Department of Pathology, University of Washington, Seattle, WA, USA 
 Department of Chemistry, University of Washington, Seattle, WA, USA 
 Department of Immunology, University of Washington, Seattle, WA, USA 
 Applied Scientific Instrumentation, Eugene, OR, USA 
 Allen Institute for Brain Science, Seattle, WA, USA 
 Department of Chemistry, University of Washington, Seattle, WA, USA; Department of Physiology and Biophysics, University of Washington, Seattle, WA, USA 
 Department of Mechanical Engineering, University of Washington, Seattle, WA, USA; Department of Pathology, University of Washington, Seattle, WA, USA 
Pages
1-8
Publication year
2019
Publication date
Jul 2019
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2252263236
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.