Abstract

Studies on vesicle formation by the Coat Protein I (COPI) complex have contributed to a basic understanding of how vesicular transport is initiated. Phosphatidic acid (PA) and diacylglycerol (DAG) have been found previously to be required for the fission stage of COPI vesicle formation. Here, we find that PA with varying lipid geometry can all promote early fission, but only PA with shortened acyl chains promotes late fission. Moreover, diacylglycerol (DAG) acts after PA in late fission, with this role of DAG also requiring shorter acyl chains. Further highlighting the importance of the short-chain lipid geometry for late fission, we find that shorter forms of PA and DAG promote the vesiculation ability of COPI fission factors. These findings advance a general understanding of how lipid geometry contributes to membrane deformation for vesicle fission, and also how proteins and lipids coordinate their actions in driving this process.

Details

Title
The late stage of COPI vesicle fission requires shorter forms of phosphatidic acid and diacylglycerol
Author
Park, Seung-Yeol 1 ; Jia-Shu, Yang 2 ; Li, Zhen 3 ; Deng, Pan 4   VIAFID ORCID Logo  ; Zhu, Xiaohong 3 ; Young, David 2 ; Ericsson, Maria 5 ; Andringa, Ruben L H 6 ; Minnaard, Adriaan J 6 ; Zhu, Chunmei 7 ; Sun, Fei 8   VIAFID ORCID Logo  ; D Branch Moody 2   VIAFID ORCID Logo  ; Morris, Andrew J 4   VIAFID ORCID Logo  ; Fan, Jun 9 ; Hsu, Victor W 2 

1  Division of Rheumatology, Immunology and Allergy, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA; Department of Medicine, Harvard Medical School, Boston, MA, USA; Department of Life Sciences, Pohang University of Science and Technology, Pohang, Gyeongbuk, Republic of Korea 
2  Division of Rheumatology, Immunology and Allergy, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA; Department of Medicine, Harvard Medical School, Boston, MA, USA 
3  Department of Materials Science and Engineering, City University of Hong Kong, Hong Kong, China 
4  Division of Cardiovascular Medicine, Department of Medicine, University of Kentucky, Lexington, KY, USA; Lexington Veterans Affairs Medical Center, Lexington, KY, USA 
5  Department of Cell Biology, Harvard Medical School, Boston, MA, USA 
6  Stratingh Institute for Chemistry, University of Groningen, Groningen, The Netherlands 
7  National Key Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China 
8  National Key Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China; Center for Biological Imaging, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China 
9  Department of Materials Science and Engineering, City University of Hong Kong, Hong Kong, China; City University of Hong Kong, Shenzhen Research Institute, Shenzhen, China 
Pages
1-14
Publication year
2019
Publication date
Jul 2019
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-019-11324-4
ProQuest document ID
2266989885
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.