Abstract

Gravitational stress in general and microgravity (µg) in particular are regarded as major stress factors responsible for immune system dysfunction in space. To assess the effects of alternating µg and hypergravity (hyper-g) on immune cells, the attachment of peripheral blood mononuclear cells (PBMCs) to adhesion molecules under flow conditions and the antigen-induced immune activation in whole blood were investigated in parabolic flight (PF). In contrast to hyper-g (1.8 g) and control conditions (1 g), flow and rolling speed of PBMCs were moderately accelerated during µg-periods which were accompanied by a clear reduction in rolling rate. Whole blood analyses revealed a “primed” state of monocytes after PF with potentiated antigen-induced pro-inflammatory cytokine responses. At the same time, concentrations of anti-inflammatory cytokines were increased and monocytes displayed a surface molecule pattern that indicated immunosuppression. The results suggest an immunologic counterbalance to avoid disproportionate immune responses. Understanding the interrelation of immune system impairing and enhancing effects under different gravitational conditions may support the design of countermeasures to mitigate immune deficiencies in space.

Details

Title
Cells´ Flow and Immune Cell Priming under alternating g-forces in Parabolic Flight
Author
Moser, D 1   VIAFID ORCID Logo  ; Sun, S J 2 ; N Li 2 ; Biere, K 1 ; Hoerl, M 1 ; Matzel, S 1 ; Feuerecker, M 1 ; J-I Buchheim 1 ; Strewe, C 1 ; Thiel, C S 3 ; Gao, Y X 2 ; Wang, C Z 2 ; Ullrich, O 3 ; Long, M 2 ; Choukèr, A 1 

 Laboratory of Translational Research “Stress and Immunity”, Department of Anaesthesiology, University Hospital, LMU Munich, Munich, Germany 
 Key Laboratory of Microgravity (National Microgravity Laboratory), Center of Biomechanics and Bioengineering, and Beijing Key Laboratory of Engineered Construction and Mechanobiology, Institute of Mechanics, Chinese Academy of Sciences, Beijing, China; School of Engineering Sciences, University of Chinese Academy of Sciences, Beijing, China 
 Institute of Anatomy, Faculty of Medicine, University of Zurich, Zurich, Switzerland; Department of Machine Design, Engineering Design and Product Development (IMK), Otto-von-Guericke-University Magdeburg, Magdeburg, Germany 
Pages
1-11
Publication year
2019
Publication date
Aug 2019
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2268064323
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.