Abstract

Preparation of sophisticated delivery systems for nanomedicine applications generally involve multi-step procedures using organic solvents. In this study, we have developed a simple self-assembling process to prepare docetaxel-loaded hyaluronic acid (HA) nanocapsules by using a self-emulsification process without the need of organic solvents, heat or high shear forces. These nanocapsules, which comprise an oily core and a shell consisting of an assembly of surfactants and hydrophobically modified HA, have a mean size of 130 nm, a zeta potential of −20 mV, and exhibit high docetaxel encapsulation efficiency. The nanocapsules exhibited an adequate stability in plasma. Furthermore, in vitro studies performed using A549 lung cancer cells, showed effective intracellular delivery of docetaxel. On the other hand, blank nanocapsules showed very low cytotoxicity. Overall, these results highlight the potential of self-emulsifying HA nanocapsules for intracellular drug delivery.

Details

Title
Self-assembled hyaluronan nanocapsules for the intracellular delivery of anticancer drugs
Author
Cadete, Ana 1 ; Olivera, Ana 1 ; Besev, Magnus 2 ; Dhal, Pradeep K 2 ; Gonçalves, Lídia 3   VIAFID ORCID Logo  ; Almeida, António J 3 ; Bastiat, Guillaume 4 ; Benoit, Jean-Pierre 4 ; de la Fuente, María 5 ; Garcia-Fuentes, Marcos 1   VIAFID ORCID Logo  ; Alonso, María José 1 ; Torres, Dolores 6 

1  Nanobiofar Group, IDIS, CIMUS, University of Santiago de Compostela, Santiago de Compostela, Spain; Department of Pharmaceutics and Pharmaceutical Technology, School of Pharmacy, University of Santiago de Compostela, Santiago de Compostela, Spain 
2  Sanofi-Global R&D, Sanofi US, Waltham, USA 
3  Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Lisbon, Portugal 
4  Micro et Nanomedecines Translationnelles, MINT, Université Angers, INSERM 1066, CNRS 6021, 4 rue Larrey, Angers, France 
5  Nano-Oncology Unit, Translational Medical Oncology Group, Health Research Institute of Santiago de Compostela (IDIS), SERGAS, Santiago de Compostela, Spain; Cancer Network Research (CIBERONC), Madrid, Spain 
6  Department of Pharmaceutics and Pharmaceutical Technology, School of Pharmacy, University of Santiago de Compostela, Santiago de Compostela, Spain 
Pages
1-11
Publication year
2019
Publication date
Aug 2019
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-019-47995-8
ProQuest document ID
2270514253
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.