Abstract

RNA interference (RNAi) has potential advantages over other gene therapy approaches due to its high specificity and the ability to inhibit target gene expression. However, the stability and tissue-specific delivery of siRNA remain as the biggest obstacles for RNAi therapeutics. Here, we developed such a system by conjugating gelatin-based nanogels with the nucleolin-targeted AS1411 aptamer and deoxynucleotide-substituted siRNA together (Apt-GS/siRNA) via a disulfide linker to achieve transient docking of siRNA. These Apt-GS/siRNA nanogels demonstrated favorable release of siRNA under reducing conditions owing to disulfide cleavage. Furthermore, this smart system could electively release siRNA into the cytosol in nucleolin-positive cells (A549) by a glutathione-triggered disassembly and subsequently efficient RNAi for luciferase. Besides, disulfide-equipped Apt-GS nanogels showed good biocompatibility in vitro. Taken together, this redox-responsive, tumor-targeting smart nanogels display great potential in exploiting functionalized siRNA delivery and tumor therapy.

Details

Title
Redox-Sensitive Gelatin/Silica-Aptamer Nanogels for Targeted siRNA Delivery
Author
Zhao, Xueqin 1   VIAFID ORCID Logo  ; Xi, Yinyin 1 ; Zhang, Yongming 1 ; Wu, Qiuyan 1 ; Meng, Ruiyuan 1 ; Zheng, Bin 2 ; Lei Rei 3 

1  College of Life Science and Medicine, Zhejiang Sci-Tech University, Hangzhou, People’s Republic of China 
2  Department of Otolaryngology, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, People’s Republic of China 
3  Department of Biomaterials, College of Materials, Xiamen University, Xiamen, People’s Republic of China 
Pages
1-9
Publication year
2019
Publication date
Aug 2019
Publisher
Springer Nature B.V.
ISSN
19317573
e-ISSN
1556276X
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/s11671-019-3101-0
ProQuest document ID
2273097446
Copyright
Nanoscale Research Letters is a copyright of Springer, (2019). All Rights Reserved., © 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.