Abstract

Numerous commercial organic fluorophores with excellent optical properties are precluded from live-cell superresolution imaging due to poor cell permeability. Here, we develop a simple but effective strategy that renders cells permeable to cell-impermeable, organic fluorescent probes by using a novel peptide vehicle, PV-1. By simple coincubation with PV-1, 22 different cell-impermeable, organic fluorescent probes were efficiently delivered into live cells and specifically labeled a variety of organelles. Moreover, PV-1 can simultaneously transfer up to three different probes into live cells. By using PV-1 and these cell-impermeable fluorescent probes, we obtained multicolor, long-term, live-cell superresolution images of various organelles, which allowed us to study the dynamic interactions between them. PV-1, together with these organic fluorescent probes, will greatly broaden the applications of superresolution imaging technology in diverse live-cell studies and opens up a new avenue in the design and application of peptide vehicles.

Details

Title
Simple and efficient delivery of cell-impermeable organic fluorescent probes into live cells for live-cell superresolution imaging
Author
Zhang, Meng 1   VIAFID ORCID Logo  ; Li, Meihua 1 ; Zhang, Wenting 1 ; Han, Yubing 1 ; Yu-Hui, Zhang 1 

1  Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei, China; MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei, China 
Pages
1-11
Publication year
2019
Publication date
Aug 2019
Publisher
Springer Nature B.V.
e-ISSN
20477538
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41377-019-0188-0
ProQuest document ID
2273183392
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.