Abstract

Debilitating cases of tendon pain and degeneration affect the majority of diabetic individuals. The high rate of tendon degeneration persists even when glucose levels are well controlled, suggesting that other mechanisms may drive tendon degeneration in diabetic patients. The purpose of this study was to investigate the impact of advanced glycation end-products on tendon fibroblasts to further our mechanistic understanding of the development and progression of diabetic tendinopathy. We proposed that advanced glycation end-products would induce limitations to mitochondrial function and proliferative capacity in tendon-derived fibroblasts, restricting their ability to maintain biosynthesis of tendon extracellular matrix. Using an in-vitro cell culture system, rat Achilles tendon fibroblasts were treated with glycolaldehyde-derived advanced glycation end-products (0, 50, 100, and 200 μg/ml) for 48 hours in normal glucose (5.5 mM) and high glucose (25 mM) conditions. We demonstrate that tendon fibroblasts treated with advanced glycation end-products display reduced ATP production, electron transport efficiency, and proliferative capacity. These impairments were coupled with alterations in mitochondrial DNA content and expression of genes associated with extracellular matrix remodeling, mitochondrial energy metabolism, and apoptosis. Our findings suggest that advanced glycation end-products disrupt tendon fibroblast homeostasis and may be involved in the development and progression of diabetic tendinopathy.

Details

Title
Advanced Glycation End-Products Suppress Mitochondrial Function and Proliferative Capacity of Achilles Tendon-Derived Fibroblasts
Author
Patel, Shivam H 1 ; Feng, Yue 2 ; Saw, Shannon K 1 ; Foguth, Rachel 3 ; Cannon, Jason R 3 ; Shannahan, Jonathan H 4 ; Kuang, Shihuan 2   VIAFID ORCID Logo  ; Sabbaghi, Arman 5 ; Carroll, Chad C 6 

1  Department of Health and Kinesiology, Purdue University, West Lafayette, IN, USA 
2  Department of Animal Sciences, Purdue University, West Lafayette, IN, USA 
3  School of Health Sciences, Purdue University, West Lafayette, IN, USA; Purdue Institute for Integrative Neuroscience, West Lafayette, IN, USA 
4  School of Health Sciences, Purdue University, West Lafayette, IN, USA 
5  Department of Statistics, Purdue University, West Lafayette, IN, USA 
6  Department of Health and Kinesiology, Purdue University, West Lafayette, IN, USA; Indiana Center for Musculoskeletal Health, Indiana University School of Medicine, Indianapolis, IN, USA 
Pages
1-17
Publication year
2019
Publication date
Aug 2019
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-019-49062-8
ProQuest document ID
2283259105
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.