Abstract

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a global threat to wheat production. Aegilops tauschii, one of the wheat progenitors, carries the YrAS2388 locus for resistance to Pst on chromosome 4DS. We reveal that YrAS2388 encodes a typical nucleotide oligomerization domain-like receptor (NLR). The Pst-resistant allele YrAS2388R has duplicated 3’ untranslated regions and is characterized by alternative splicing in the nucleotide-binding domain. Mutation of the YrAS2388R allele disrupts its resistance to Pst in synthetic hexaploid wheat; transgenic plants with YrAS2388R show resistance to eleven Pst races in common wheat and one race of P. striiformis f. sp. hordei in barley. The YrAS2388R allele occurs only in Ae. tauschii and the Ae. tauschii-derived synthetic wheat; it is absent in 100% (n = 461) of common wheat lines tested. The cloning of YrAS2388R will facilitate breeding for stripe rust resistance in wheat and other Triticeae species.

Details

Title
An ancestral NB-LRR with duplicated 3′UTRs confers stripe rust resistance in wheat and barley
Author
Zhang, Chaozhong 1   VIAFID ORCID Logo  ; Huang, Lin 2 ; Zhang, Huifei 3 ; Qunqun Hao 3 ; Lyu, Bo 3 ; Wang, Meinan 4 ; Epstein, Lynn 5   VIAFID ORCID Logo  ; Liu, Miao 2   VIAFID ORCID Logo  ; Kou, Chunlan 2 ; Qi, Juan 6   VIAFID ORCID Logo  ; Chen, Fengjuan 6 ; Li, Mengkai 6 ; Gao, Ge 6 ; Ni, Fei 6   VIAFID ORCID Logo  ; Zhang, Lianquan 2   VIAFID ORCID Logo  ; Hao, Ming 2 ; Wang, Jirui 2 ; Chen, Xianming 7   VIAFID ORCID Logo  ; Ming-Cheng, Luo 8   VIAFID ORCID Logo  ; Zheng, Youliang 2 ; Wu, Jiajie 6   VIAFID ORCID Logo  ; Liu, Dengcai 9   VIAFID ORCID Logo  ; Fu, Daolin 3   VIAFID ORCID Logo 

1  State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, Shandong, China; Department of Plant Sciences, University of Idaho, Moscow, ID, USA 
2  Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China 
3  Department of Plant Sciences, University of Idaho, Moscow, ID, USA 
4  Department of Plant Pathology, Washington State University, Pullman, WA, USA 
5  Department of Plant Pathology, University of California, Davis, CA, USA 
6  State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, Shandong, China 
7  Wheat Health, Genetics, and Quality Research Unit, USDA-ARS, Pullman, WA, USA 
8  Department of Plant Sciences, University of California, Davis, CA, USA 
9  Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan, China 
Pages
1-12
Publication year
2019
Publication date
Sep 2019
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-019-11872-9
ProQuest document ID
2285699224
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.