Full text

Turn on search term navigation

© 2013. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Lignocellulosic biomass, if properly saccharified, could be an ideal feedstock for biohydrogen production. However, the high cellulases cost is the key obstacle to its development. In this work, cost-effective enzyme produced by Trichoderma viride was used to saccharify cornstalk. To obtain high sugar yield, a central composite design of response surface method was used to optimize enzymatic saccharification process. Experimental results showed that the enzymatic saccharification rate reached the highest of 81.2% when pH, temperature, cellulases and substrate concentration were 5, 49.7 °C, 35.7 IU g−1, and 38.5 g L−1, respectively. The cornstalk hydrolysate was subsequently introduced to fermentation by Thermoanaerobacterium thermosaccharolyticum W16, the yield of hydrogen reached the highest level of 90.6 ml H2 g−1 pretreated cornstalk. The present results indicate the potential of using T. thermosaccharolyticum W16 for high yield conversion of cornstalk hydrolysate, which was saccharified by onsite enzyme produced by T. viride.

Details

Title
Enzymatic saccharification of cornstalk by onsite cellulases produced by Trichoderma viride for enhanced biohydrogen production
Author
Zhao, Lei 1 ; Guang-Li Cao 2 ; Ai-Jie, Wang 1 ; Hong-Yu, Ren 1 ; Cheng-Jiao, Xu 1 ; Nan-Qi Ren 1 

 State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China 
 State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China; School of Life Science and Technology, Harbin Institute of Technology, Harbin, China 
Pages
591-598
Section
Technical Advance
Publication year
2013
Publication date
Sep 2013
Publisher
John Wiley & Sons, Inc.
ISSN
17571693
e-ISSN
17571707
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2287890163
Copyright
© 2013. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.