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© 2013. This work is published under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

The opportunistic pathogen Pseudomonas aeruginosa utilizes type III secretion system (T3SS) to translocate effector proteins into eukaryotic host cells that subvert normal host cell functions to the benefit of the pathogen, and results in serious infections. T3SS in P. aeruginosa is controlled by a complex system of regulatory mechanisms and signaling pathways. In this study, we described that Crc, an RNA‐binding protein, exerts a positive impact on T3SS in P. aeruginosa, as evidenced by promoter activity assays of several key T3SS genes, transcriptomics, RTPCR, and immunoblotting in crc mutant. We further demonstrated that the regulatory function of Crc on the T3SS was mediated through the T3SS master regulator ExsA and linked to the Cbr/Crc signaling system. Expression profiling of the crc mutant revealed a downregulation of flagship T3SS genes as well as 16 other genes known to regulate T3SS gene expression in P. aeruginosa. On the basis of these data, we proposed that Crc may exert multifaceted control on the T3SS through various pathways, which may serve to fine‐tune this virulence mechanism in response to environmental changes and nutrient sources.

Details

Title
The global regulator Crc plays a multifaceted role in modulation of type III secretion system in P seudomonas aeruginosa
Author
Yi‐Hu Dong 1 ; Xi‐Fen Zhang 1 ; Lian‐Hui Zhang 1 

 Institute of Molecular and Cell Biology, Singapore 
Pages
161-172
Section
Original Research
Publication year
2013
Publication date
Feb 2013
Publisher
John Wiley & Sons, Inc.
e-ISSN
20458827
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2289849258
Copyright
© 2013. This work is published under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.