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Abstract
The miRNA profile of mesenchymal stem cells (MSCs) derived from amniotic fluid, bone marrow (BM), and umbilical cord blood was analyzed. Initially, 67 different miRNAs were identified that were expressed in all three types of MSCs but at different levels, depending on the source. A more detailed analysis revealed that miR-21 was expressed at higher levels in RS-AF-MSCs and BM-MSCs compared with SS-AF-MSCs. Findings suggest that miR-21 might function by regulating Sox2 expression in human MSCs and might also act as a key molecule determining MSC proliferation and differentiation.
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Details
1 Laboratory of Biology, School of Medicine, University of Athens, Athens, Greece; Biotechnology Laboratory, Center of Basic Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece
2 Laboratory of Biology, School of Medicine, University of Athens, Athens, Greece; First Department of Obstetrics and Gynecology, School of Medicine, University of Athens, Athens, Greece
3 Cell and Gene Therapy Laboratory, Center of Basic Research II