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© 2013. This work is published under http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Premise of the study: Three species of the mangrove tree genus Rhizophora are found in the New World and along the west coast of Africa. Of these, R. mangle is the most abundant and has a complex interbreeding relationship with the sympatric R. racemosa and R. harrisonii. The development of additional microsatellite markers would permit paternity analyses and investigation of the hybrid origin of these species.

Methods and Results: Using an enriched library method, via hybridization with biotinylated oligonucleotides complementary to repetitive poly AG/TC, primers for 11 microsatellite markers of R. mangle were developed and characterized in populations in Pará and São Paulo (Brazil) and Florida (USA). Ten of these markers were transferable to R. racemosa and R. harrisonii.

Conclusions: The microsatellite markers presented here will be useful in studies of contemporary and historical gene flow between American and West African Rhizophora species.

Details

Title
Isolation of microsatellite markers for the red mangrove, Rhizophora mangle (Rhizophoraceae)
Author
Ribeiro, Diana O 1 ; Vinson, Christina C 2 ; Nascimento, Dulcivania S S 1 ; Mehlig, Ulf 1 ; Menezes, Moirah P M 1 ; Sampaio, Iracilda 1 ; Silva, Marivana B 1 

 Institute for Coastal Research, Universidade Federal do Pará (UFPA), Alameda Leandro Ribeiro s/n, Aldeia, 68600‐000 Bragança, Pará, Brazil 
 Molecular Biology and Biochemistry, Universidade Federal de Viçosa, Viçosa, 36570‐000 Minas Gerais, Brazil 
Section
Primer Notes
Publication year
2013
Publication date
Sep 2013
Publisher
John Wiley & Sons, Inc.
e-ISSN
21680450
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2290141218
Copyright
© 2013. This work is published under http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.