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© 2013. This work is published under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Due to their unique properties, engineered nanoparticles (NPs) have found broad use in industry, technology, and medicine, including as a vehicle for drug delivery. However, the understanding of NPs’ interaction with different types of mammalian cells lags significantly behind their increasing adoption in drug delivery. In this study, we show unique responses of human epithelial breast cells when exposed to polymeric Eudragit® RS NPs (ENPs) for 1–3 days. Cells displayed dose‐dependent increases in metabolic activity and growth, but lower proliferation rates, than control cells, as evidenced in tetrazolium salt (WST‐1) and 5‐bromo‐2′‐deoxyuridine (BrdU) assays, respectively. Those effects did not affect cell death or mitochondrial fragmentation. We attribute the increase in metabolic activity and growth of cells culture with ENPs to three factors: (1) high affinity of proteins present in the serum for ENPs, (2) adhesion of ENPs to cells, and (3) activation of proliferation and growth pathways. The proteins and genes responsible for stimulating cell adhesion and growth were identified by mass spectrometry and Microarray analyses. We demonstrate a novel property of ENPs, which act to increase cell metabolic activity and growth and organize epithelial cells in the epithelium as determined by Microarray analysis.

Details

Title
Unique growth pattern of human mammary epithelial cells induced by polymeric nanoparticles
Author
Hussien, Rajaa 1 ; Rihn, Bertrand H 2 ; Eidi, Housam 2 ; Ronzani, Carole 2 ; Joubert, Olivier 2 ; Ferrari, Luc 2 ; Vazquez, Oscar 3 ; Kaufer, Daniela 3 ; Brooks, George A 1 

 Department of Integrative Biology, University of California, Berkeley, California 
 Faculté de Pharmacie, EA 3452 CITHEFOR, Nancy‐Université, 54001 Nancy Cedex, France 
 Helen Wills Neuroscience Institute, University of California, Berkeley, California 
Section
Original Research
Publication year
2013
Publication date
Sep 2013
Publisher
John Wiley & Sons, Inc.
e-ISSN
2051817X
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2290533030
Copyright
© 2013. This work is published under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.