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Introduction
In this study antioxidant properties of the leaf extract has been studied. Antioxidants counteract oxidative stress created by free radicals. A Free radical is any molecule that contains an unpaired electron in an atomic orbital and they are unstable and highly reactive by donating or accepting an electron from other molecules, thereby behave as oxidants or reductants (Cheeseman et al., 1998) They cause damage to biological relevant molecules such as DNA, proteins, carbohydrates, and lipids [Young et al., 2001] Cardiovascular disorder, cancer, osteoporosis and degenerative disease caused by the production of reactive oxygen species and oxidative stress (Halliwell et al., 1994). Unsaturated fatty acid induced by free radicals in the membranes, leads to membrane lipid peroxidation, decrease the membrane fluidity, reduction of enzyme receptor activity and damage to membrane protein, which finally triggers the cell inactivation or death (Li.X.M et al., 2007).
Oxidative processes maintained by antioxidants protect the cell from oxidative stress or it creates reactive metabolites Antioxidants act as a blocker or react against free radical to inhibit the toxic effect, scavenge and destroy the physiological system of free radical. Natural antioxidant explored from medicinal plants would be a replacement of synthetic antioxidants, which were restricted due to side effect such as carcinogenicity.The present study focused onPlumbagozeylanicaleave extract usingfour different solvents based on polarity and its antioxidant activity.
Material and Methods
Solvent Extraction of Plant
The leaf of P.zeylanica collected from homoeopathic store, wash the leaf and Shade dried for 15 days. Once grind the dried leaf, 100g of leaf powder combined with 200ml solvents as per polarity (Hexane, Chloroform, Ethyl acetate and Methanol) and blend the mixture to placed on orbital shaker at room temperature for 72 hours. Afterward collect the filtrate by what mann no-1 filter paper, then extract were concentrated to evaporate the solvent by rotary evaporator at low temperature under medium pressure condition. The dried crude extract was stored in airtight container for further experiment and analysis.
In Vitro Antioxidant Activity Assay
Antioxidant Activity by DPPH Assay
Free radical scavenging activity of various plant extracts is measured by the DPPH assay method. DPPH (1,1-Diphenyl-2-picrylhydrazyl) is a stable free radical, which is purple in color and its observance is measured at 517nm. Antioxidant present in the plant...