Abstract

Objective

Gastrointestinal tract lymphomas are currently detected more frequently due to advances in endoscopic technology. The aim of this study was to assess the feasibility of flow cytometric analysis of restricted light chain in endoscopic biopsy specimens for the diagnosis of gastrointestinal tract B-cell lymphoma. We prepared viable cell suspensions from unfixed specimens obtained from 10 consecutive patients who had a previous histological diagnosis of gastrointestinal tract B-cell lymphoma. We performed immunophenotypic studies with multi-color flow cytometry and assessed clonality through examination of immunoglobulin light chain expression exclusively in a population identified by anti-CD45 or CD20 antibodies.

Results

We could perform light chain expression analysis with 2 endoscopic biopsy specimens from all 10 patients with gastrointestinal tract B-cell lymphoma. We conclude that flow cytometric analysis of endoscopic biopsy specimens is feasible and thus likely useful for the diagnosis of gastrointestinal tract B-cell lymphoma in clinical settings.

Trial registration UMIN Clinical Trials Registry, UMIN000027730. Registered 12 June 2017

Details

Title
Feasibility of flow cytometric analysis of restricted light chain in endoscopic biopsy specimens from patients with gastrointestinal tract B cell lymphoma: a pilot study
Author
Matsueda, Katsunori; Iwamuro, Masaya; Takahashi, Takahide; Omote, Sizuma; Nishida, Kenji; Tanaka, Takehiro; Ennishi, Daisuke; Otsuka, Fumio; Yoshino, Tadashi; Okada, Hiroyuki
Section
Research note
Publication year
2019
Publication date
2019
Publisher
BioMed Central
e-ISSN
17560500
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/s13104-019-4578-4
ProQuest document ID
2293276410
Copyright
© 2019. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.