Abstract

Background

Porcine circovirus type 3 (PCV3) is an emerging circovirus species, that has been reported in major pig-raising countries including the United States, China, South Korea, Brazil, Spain, and Poland.

Results

A real-time loop-mediated isothermal amplification (LAMP) assay was developed for rapid detection of porcine circovirus 3 (PCV3). The method had a detection limit of 1 × 101 copies/μL with no cross-reactions with classical swine fever virus (CSFV) C strain, foot-and-mouth disease virus (FMDV), porcine circovirus 2 (PCV2) LG vaccine strain, porcine epidemic diarrhoea virus (PEDV), porcine respiratory and reproductive syndrome virus (PRRSV), or pseudorabies virus (PRV). The PCV3 positive detection rate of 203 clinical samples for the real-time LAMP assay was 89.66% (182/203).

Conclusions

The real-time LAMP assay is highly sensitive, and specific for use in epidemiological investigations of PCV3.

Details

Title
Development of a real-time loop-mediated isothermal amplification assay for detection of porcine circovirus 3
Author
Wang, Huanan; Liu, Xiangnan; Zeng, Fanwen; Zhang, Tongyuan; Lian, Yuexiao; Wu, Miaoli; Li, Xiao; Zhu, Yujun; Zhang, Yu; Chen, Meili; Huang, Ren; Luo, Manlin; Feng Cong; Guo, Pengju
Section
Methodology article
Publication year
2019
Publication date
2019
Publisher
BioMed Central
e-ISSN
17466148
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/s12917-019-2037-z
ProQuest document ID
2293443295
Copyright
© 2019. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.