Abstract

Serum and tissue miR-21 expression in patients with breast cancer (BC) is a useful biomarker for cancer diagnosis, progression, and treatment. Matrix metalloproteinase-1 (MMP-1) is also important in breast cancer carcinogenesis. However, miR-21 and MMP-1/CD63 in urine exosomes in these patients have not been examined. Urine samples were collected from patients with BC and 26 healthy females. Urinary exosomes were isolated and confirmed by western blotting with anti-CD63 antibody and electron microscopy observation. MiR-21 and MMP-1/CD63 expression was examined by quantitative RT-PCR and western blotting, respectively. Patients with very early stage breast cancer were evaluated. MiR-21 expression in the patients was 0.26 [95% CI: 0.20–0.78], which was significant lower than in the 26 controls (1.00 [95% CI: 1.01–3.37], p = 0.0947). MMP-1/CD63 expression in patients was significantly higher than in controls (1.74 [95% CI: 0.86–5.08] vs 0.535 [95% CI: −0.01–2.81], p = 0.0001). Sensitivity and specificity were 0.708 and 0.783 for miR-21 and 0.792 and 0.840 for MMP-1/CD63, respectively. Sensitivity and specificity of combined expression were 95% and 79%, respectively. The sensitivity of MMP-1/CD63 expression in urinary exosomes was better than that of miR-21 expression. Thus, miR-21 and MMP/CD63 may be useful markers for BC screening.

Details

Title
Novel breast cancer screening: combined expression of miR-21 and MMP-1 in urinary exosomes detects 95% of breast cancer without metastasis
Author
Ando, Wataru 1   VIAFID ORCID Logo  ; Kikuchi, Kiyoshi 2 ; Uematsu, Takayuki 3   VIAFID ORCID Logo  ; Yokomori, Hiroaki 4 ; Takaki, Takashi 5 ; Sogabe, Masaya 6 ; Kohgo, Yutaka 7 ; Otori, Katsuya 1 ; Ishikawa, Shigemi 8 ; Okazaki, Isao 9 

1  Department of Clinical Pharmacy, Center for Clinical Pharmacy and Sciences, Kitasato University School of Pharmacy, Minato-ku, Tokyo, Japan 
2  Department of Surgery, Sanno Hospital, International University of Health and Welfare, Minato-ku, Tokyo, Japan 
3  Biomedical Laboratory, Division of Biomedical Research, Kitasato University Medical Center, Kitamoto City, Saitama, Japan 
4  Department of Internal Medicine, Kitasato University Medical Center, Kitamoto City, Saitama, Japan 
5  Division of Electron microscopy, Showa University School of Medicine, Shinagawa-ku, Tokyo, Japan 
6  Department of General Thoracic Surgery, Jichi Medical University, Shimotsuke, Tochigi, Japan 
7  Department of Internal Medicine, International University of Health and Welfare Hospital, Nasu-Shiobara, Tochigi, Japan; Health Care Center, International University of Health and Welfare Hospital, Nasu-Shiobara, Tochigi, Japan 
8  Department of Chest Surgery, International University of Health and Welfare Hospital, Nasu-Shiobara, Tochigi, Japan 
9  Department of Internal Medicine, International University of Health and Welfare Hospital, Nasu-Shiobara, Tochigi, Japan; Health Care Center, International University of Health and Welfare Hospital, Nasu-Shiobara, Tochigi, Japan; Department of Internal Medicine, Sanno Hospital, International University of Health and Welfare, Minato-ku, Tokyo, Japan 
Pages
1-10
Publication year
2019
Publication date
Sep 2019
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-019-50084-5
ProQuest document ID
2293851334
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.