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Copyright © 2019 Yu Zhang et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. http://creativecommons.org/licenses/by/4.0/

Abstract

The present study investigated the antiapoptotic and antigenotoxic capabilities of N-acetyl cysteine- (NAC-) containing polymethyl methacrylate (PMMA) resin. An in vitro Transwell insert model was used to mimic the clinical provisional restorations placed on vital teeth. Various parameters associated with cell apoptosis and genotoxicity were investigated to obtain a deeper insight into the underlying mechanisms. The exposure of human dental pulp cell (hDPC) cultures to the PMMA resin (Unifast Trad™) resulted in a rapid increase in reactive oxygen species (ROS) level beginning at 1 h, which was followed by time-dependent cell detachment and overt death. The formation of γ-H2AX and cell cycle G1 phase arrest indicated that oxidative DNA damage occurred as a result of the interactions between DNA bases and ROS, beyond the capacities of cellular redox regulation. Such oxidative DNA damage triggers the activation of p53 via the ataxia telangiectasia mutated (ATM) signaling pathway and the induction of intrinsic mitochondrial apoptosis. Oxidative stress, cell apoptosis, and DNA damage induced by the PMMA resin were recovered to almost the level of untreated controls by the incorporation of NAC. The results indicate that the PMMA resin induced the intrinsic mitochondrial apoptosis as a consequence of p53 activation via the ATM pathway in response to oxidative DNA damage. More importantly, the incorporation of NAC as a novel component into the Unifast Trad™ PMMA resin offers protective effects against cell apoptosis and genotoxicity. This procedure represents a beneficial strategy for developing more biocompatible PMMA-based resin materials.

Details

Title
N-Acetyl Cysteine as a Novel Polymethyl Methacrylate Resin Component: Protection against Cell Apoptosis and Genotoxicity
Author
Zhang, Yu 1 ; Jian-feng, Xiao 1 ; He-feng, Yang 1 ; Jiao, Yang 2   VIAFID ORCID Logo  ; Wei-wei, Cao 3 ; Huan-min Shi 1 ; Jing-fen Cun 1 ; Tay, Franklin R 4   VIAFID ORCID Logo  ; Ping, Jie 5   VIAFID ORCID Logo  ; Yu-hong, Xiao 6   VIAFID ORCID Logo 

 The Affiliated Stomatological Hospital of Kunming Medical University, Kunming, China 
 Department of Stomatology, The 7th Medical Center of PLA General Hospital, Beijing, China 
 Freetech Technology, Nanjing, China 
 Department of Endodontics, The Dental College of Georgia, Augusta University, Augusta, GA, USA 
 Department of Medical Administration, The 7th Medical Center of PLA General Hospital, Beijing, China 
 Department of Stomatology, 920th Hospital of Joint Logistics Support Force, Kunming, China 
Editor
Manuela Curcio
Publication year
2019
Publication date
2019
Publisher
John Wiley & Sons, Inc.
ISSN
19420900
e-ISSN
19420994
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2299079717
Copyright
Copyright © 2019 Yu Zhang et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. http://creativecommons.org/licenses/by/4.0/