Abstract

In plants, changes in local auxin concentrations can trigger a range of developmental processes as distinct tissues respond differently to the same auxin stimulus. However, little is known about how auxin is interpreted by individual cell types. We performed a transcriptomic analysis of responses to auxin within four distinct tissues of the Arabidopsis thaliana root and demonstrate that different cell types show competence for discrete responses. The majority of auxin-responsive genes displayed a spatial bias in their induction or repression. The novel data set was used to examine how auxin influences tissue-specific transcriptional regulation of cell-identity markers. Additionally, the data were used in combination with spatial expression maps of the root to plot a transcriptomic auxin-response gradient across the apical and basal meristem. The readout revealed a strong correlation for thousands of genes between the relative response to auxin and expression along the longitudinal axis of the root. This data set and comparative analysis provide a transcriptome-level spatial breakdown of the response to auxin within an organ where this hormone mediates many aspects of development.

Details

Title
A map of cell type-specific auxin responses
Author
Bargmann, Bastiaan O R 1 ; Vanneste, Steffen 2 ; Krouk, Gabriel 3 ; Nawy, Tal 4 ; Efroni, Idan 4 ; Shani, Eilon 5 ; Choe, Goh 5 ; Friml, Jiří 6 ; Bergmann, Dominique C 7 ; Estelle, Mark 5 ; Birnbaum, Kenneth D 4 

 Biology Department, Center for Genomics and Systems Biology, New York University, New York, NY, USA; Department of Cell and Developmental Biology, UCSD, La Jolla, CA, USA 
 Department of Plant Systems Biology, VIB, Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium 
 Laboratoire de Biochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes—Claude Grignon, Montpellier, France 
 Biology Department, Center for Genomics and Systems Biology, New York University, New York, NY, USA 
 Department of Cell and Developmental Biology, UCSD, La Jolla, CA, USA 
 Department of Plant Systems Biology, VIB, Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium; Institute of Science and Technology Austria (IST Austria), Klosterneuburg, Austria 
 Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA 
Section
Article
Publication year
2013
Publication date
2013
Publisher
EMBO Press
e-ISSN
17444292
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2299132626
Copyright
© 2013. This work is published under http://creativecommons.org/licenses/by-nc-sa/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.