Abstract

To develop a culture model for drug development and tissue-engineering human retina, retinal pigment epithelia (RPE) were derived from human embryonic stem cells (hESCs), and their barrier properties were compared with those of a well-regarded model of RPE function, human fetal RPE isolated from 16-week-gestation fetuses (hfRPE). It was found that hESC-derived RPE is highly differentiated but may be less mature than RPE isolated from 16-week fetuses. The study also identified a panel of genes to monitor further maturation of RPE.

Details

Title
Engineering a Blood-Retinal Barrier With Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium: Transcriptome and Functional Analysis
Author
Peng Shaomin 1 ; Gan Geliang 2 ; Qiu Caihong 3 ; Zhong Mei 3 ; An, Hongyan 3 ; Adelman, Ron A 4 ; Rizzolo, Lawrence J 2 

1  Departments of Surgery; Department of Ophthalmology, Second Affiliated Hospital of Harbin Medical University, Harbin, China; Departments of Ophthalmology 
2  Departments of Surgery; Departments of Ophthalmology 
3  Departments of Cell Biology, Yale University School of Medicine, New Haven, Connecticut, USA 
4  Departments of Ophthalmology 
Pages
534-544
Section
Tissue Engineering and Regenerative Medicine
Publication year
2013
Publication date
Jul 2013
Publisher
Oxford University Press
ISSN
21576564
e-ISSN
21576580
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.5966/sctm.2012-0134
ProQuest document ID
2299132715
Copyright
© 2013. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.