Abstract

Despite recent advances in understanding microbial diversity in skin homeostasis, the relevance of microbial dysbiosis in inflammatory disease is poorly understood. Here we perform a comparative analysis of skin microbial communities coupled to global patterns of cutaneous gene expression in patients with atopic dermatitis or psoriasis. The skin microbiota is analysed by 16S amplicon or whole genome sequencing and the skin transcriptome by microarrays, followed by integration of the data layers. We find that atopic dermatitis and psoriasis can be classified by distinct microbes, which differ from healthy volunteers microbiome composition. Atopic dermatitis is dominated by a single microbe (Staphylococcus aureus), and associated with a disease relevant host transcriptomic signature enriched for skin barrier function, tryptophan metabolism and immune activation. In contrast, psoriasis is characterized by co-occurring communities of microbes with weak associations with disease related gene expression. Our work provides a basis for biomarker discovery and targeted therapies in skin dysbiosis.

Details

Title
Microbe-host interplay in atopic dermatitis and psoriasis
Author
Fyhrquist, Nanna 1   VIAFID ORCID Logo  ; Muirhead, Gareth 2 ; Prast-Nielsen, Stefanie 3   VIAFID ORCID Logo  ; Jeanmougin, Marine 4 ; Olah, Peter 5 ; Skoog, Tiina 6   VIAFID ORCID Logo  ; Jules-Clement, Gerome 4 ; Feld, Micha 7 ; Barrientos-Somarribas, Mauricio 8 ; Sinkko, Hanna 1 ; Ellen H van den Bogaard 9 ; Zeeuwen, Patrick LJM 9 ; Rikken, Gijs 9 ; Schalkwijk, Joost 9 ; Niehues, Hanna 9   VIAFID ORCID Logo  ; Däubener, Walter 10   VIAFID ORCID Logo  ; Eller, Silvia Kathrin 10 ; Alexander, Helen 11 ; Pennino, Davide 12 ; Suomela, Sari 13 ; Tessas, Ioannis 13 ; Lybeck, Emilia 13 ; Baran, Anna M 7 ; Darban, Hamid 8 ; Roopesh Singh Gangwar 14   VIAFID ORCID Logo  ; Gerstel, Ulrich 15 ; Jahn, Katharina 7 ; Karisola, Piia 16 ; Lee, Yan 17 ; Hansmann, Britta 15 ; Katayama, Shintaro 6 ; Meller, Stephan 7 ; Bylesjö, Max 18 ; Hupé, Philippe 19   VIAFID ORCID Logo  ; Levi-Schaffer, Francesca 14 ; Greco, Dario 20   VIAFID ORCID Logo  ; Ranki, Annamari 13 ; Schröder, Jens M 15   VIAFID ORCID Logo  ; Barker, Jonathan 11 ; Kere, Juha 21   VIAFID ORCID Logo  ; Tsoka, Sophia 17 ; Lauerma, Antti 13   VIAFID ORCID Logo  ; Soumelis, Vassili 22   VIAFID ORCID Logo  ; Nestle, Frank O 12 ; Homey, Bernhard 7 ; Andersson, Björn 8   VIAFID ORCID Logo  ; Alenius, Harri 1   VIAFID ORCID Logo 

1  Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden; Department of Bacteriology and Immunology, Medicum, University of Helsinki, Helsinki, Finland 
2  Department of Informatics, Faculty of Natural and Mathematical Sciences, King’s College London, London, UK; Cutaneous Medicine Unit, St. John’s Institute of Dermatology and Biomedical Research Centre, Faculty of Life Sciences and Medicine, King’s College London, London, UK 
3  Centre for Translational Microbiome Research (CTMR), Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden 
4  Institut Curie, Paris, France; INSERM, U900, Paris, France; Mines ParisTech, Fontainebleau, France; INSERM, U932, Paris, France 
5  Department of Dermatology, University Hospital Duesseldorf, Duesseldorf, Germany; Department of Dermatology, Venereology and Oncodermatology, University of Pécs, Pécs, Hungary 
6  Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden 
7  Department of Dermatology, University Hospital Duesseldorf, Duesseldorf, Germany 
8  Department of Cell and Molecular Biology, Science for Life Laboratory, Karolinska Institutet, Stockholm, Sweden 
9  Department of Dermatology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands 
10  Institute for Medical Microbiology and Hospital Hygiene, Heinrich Heine University Duesseldorf, Duesseldorf, Germany 
11  St John’s Institute of Dermatology, Division of Genetics and Molecular Medicine, Faculty of Life Sciences and Medicine, Kings College London, London, UK 
12  Cutaneous Medicine Unit, St. John’s Institute of Dermatology and Biomedical Research Centre, Faculty of Life Sciences and Medicine, King’s College London, London, UK 
13  Department of Dermatology, Allergology and Venereology, University of Helsinki and Helsinki University Hospital, Inflammation Centre, Helsinki, Finland 
14  Pharmacology Unit, School of Pharmacy, The Institute for Drug Research, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel 
15  Department of Dermatology, University Hospital Schleswig-Holstein, Kiel, Germany 
16  Department of Bacteriology and Immunology, Medicum, University of Helsinki, Helsinki, Finland 
17  Department of Informatics, Faculty of Natural and Mathematical Sciences, King’s College London, London, UK 
18  Fios Genomics, Edinburgh, UK 
19  Institut Curie, Paris, France; INSERM, U900, Paris, France; Mines ParisTech, Fontainebleau, France; CNRS, UMR144, Paris, France 
20  Faculty of Medicine and Life Sciences, University of Tampere, Tampere, Finland; Institute of Biomedical Technology, University of Tampere, Tampere, Finland; Institute of Biotechnology, University of Helsinki, Helsinki, Finland 
21  Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden; School of Basic and Medical Biosciences, King’s College London, London, UK 
22  Institut Curie, Paris, France; INSERM, U932, Paris, France 
Pages
1-15
Publication year
2019
Publication date
Oct 2019
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-019-12253-y
ProQuest document ID
2306201327
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.