Abstract

Vibrio harveyi is a major bacterial pathogen that causes fatal vibriosis in Chinese tongue sole (Cynoglossus semilaevis), resulting in massive mortality in the farming industry. However, the molecular mechanisms of C. semilaevis response to V. harveyi infection are poorly understood. Here, we performed transcriptomic analysis of C. semilaevis, comparing resistant and susceptible families in response to V. harveyi challenge (CsRC and CsSC) and control conditions (CsRU and CsSU). RNA libraries were constructed using 12 RNA samples isolated from three biological replicates of the four groups. We performed transcriptome sequencing on an Illumina HiSeq platform, and generating a total of 1,095 million paired-end reads, with the number of clean reads per library ranging from 75.27 M to 99.97 M. Through pairwise comparisons among the four groups, we identified 713 genes exhibiting significant differences at the transcript level. Furthermore, the expression levels were validated by real-time qPCR. Our results provide a valuable resource and new insights into the immune response to V. harveyi infection.

Alternate abstract:

Measurement(s)messenger RNATechnology Type(s)RNA sequencingFactor Type(s)experimental condition • Phenotypic variabilitySample Characteristic - OrganismCynoglossus semilaevisSample Characteristic - Environmentaquatic natural environment

Machine-accessible metadata file describing the reported data: https://doi.org/10.6084/m9.figshare.9907706

Details

Title
Comparative transcriptome profiling of immune response against Vibrio harveyi infection in Chinese tongue sole
Author
Xu, Hao 1 ; Xu, Xiwen 2 ; Li, Xihong 2 ; Wang, Lei 2 ; Cheng, Jiayu 3 ; Zhou, Qian 2   VIAFID ORCID Logo  ; Chen, Songlin 2 

1  Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture; Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, China; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China 
2  Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture; Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, China 
3  Tangshan Center of Engineering and Technology for flatfish Aquaculture, Weizhuo Aquaculture Co. Ltd, Tangshan, China 
Pages
1-7
Publication year
2019
Publication date
Dec 2019
Publisher
Nature Publishing Group
e-ISSN
20524463
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41597-019-0231-2
ProQuest document ID
2309512213
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.