Abstract

The search for biomarkers associated with obesity-related diseases is ongoing, but it is not clear whether plasma and serum can be used interchangeably in this process. Here we used high-throughput screening to analyze 358 proteins and 76 lipids, selected because of their relevance to obesity-associated diseases, in plasma and serum from age- and sex-matched lean and obese humans. Most of the proteins/lipids had similar concentrations in plasma and serum, but a subset showed significant differences. Notably, a key marker of cardiovascular disease PAI-1 showed a difference in concentration between the obese and lean groups only in plasma. Furthermore, some biomarkers showed poor correlations between plasma and serum, including PCSK9, an important regulator of cholesterol homeostasis. Collectively, our results show that the choice of biofluid may impact study outcome when screening for obesity-related biomarkers and we identify several markers where this will be the case.

Details

Title
Comparative analysis of obesity-related cardiometabolic and renal biomarkers in human plasma and serum
Author
Rajan, Meenu Rohini 1 ; Sotak Matus 1   VIAFID ORCID Logo  ; Barrenäs Fredrik 2 ; Shen, Tong 3 ; Borkowski Kamil 3 ; Ashton, Nicholas J 4 ; Biörserud Christina 5 ; Lindahl, Tomas L 6 ; Ramström Sofia 7 ; Schöll, Michael 8 ; Lindahl Per 9 ; Fiehn, Oliver 3   VIAFID ORCID Logo  ; Newman, John W 10   VIAFID ORCID Logo  ; Perkins, Rosie 9   VIAFID ORCID Logo  ; Wallenius Ville 11 ; Lange, Stephan 12   VIAFID ORCID Logo  ; Börgeson Emma 13   VIAFID ORCID Logo 

1  Wallenberg Laboratory, Institute of Medicine, University of Gothenburg, Department of Molecular and Clinical Medicine, Gothenburg, Sweden (GRID:grid.8761.8) (ISNI:0000 0000 9919 9582); University of Gothenburg, Wallenberg Centre for Molecular and Translational Medicine, Gothenburg, Sweden (GRID:grid.8761.8) (ISNI:0000 0000 9919 9582) 
2  Wallenberg Laboratory, Institute of Medicine, University of Gothenburg, Department of Molecular and Clinical Medicine, Gothenburg, Sweden (GRID:grid.8761.8) (ISNI:0000 0000 9919 9582); University of Gothenburg, Wallenberg Centre for Molecular and Translational Medicine, Gothenburg, Sweden (GRID:grid.8761.8) (ISNI:0000 0000 9919 9582); Uppsala University, Department of Cell & Molecular Biology, Uppsala, Sweden (GRID:grid.8993.b) (ISNI:0000 0004 1936 9457) 
3  University of California Davis, NIH West Coast Metabolomics Center, Genome Center, Davis, USA (GRID:grid.27860.3b) (ISNI:0000 0004 1936 9684) 
4  University of Gothenburg, Wallenberg Centre for Molecular and Translational Medicine, Gothenburg, Sweden (GRID:grid.8761.8) (ISNI:0000 0000 9919 9582); Institute of Physiology and Neuroscience, University of Gothenburg, Department of Psychiatry and Neurochemistry, Gothenburg, Sweden (GRID:grid.8761.8) (ISNI:0000 0000 9919 9582); Maurice Wohl Clinical Neuroscience Institute, King’s College London, Institute of Psychiatry, Psychology & Neuroscience, London, UK (GRID:grid.8761.8); NIHR Biomedical Research Centre for Mental Health & Biomedical Research Unit for Dementia at South London & Maudsley NHS Foundation, London, UK (GRID:grid.454378.9) 
5  Institute of Clinical Sciences, Sahlgrenska University Hospital, University of Gothenburg, Department of Gastrosurgical Research and Education, Gothenburg, Sweden (GRID:grid.454378.9) 
6  Linköping University, Department of Clinical Chemistry and Department of Clinical and Experimental Medicine, Linköping, Sweden (GRID:grid.5640.7) (ISNI:0000 0001 2162 9922) 
7  Linköping University, Department of Clinical Chemistry and Department of Clinical and Experimental Medicine, Linköping, Sweden (GRID:grid.5640.7) (ISNI:0000 0001 2162 9922); School of Medical Sciences, Örebro University, Cardiovascular Research Centre, Örebro, Sweden (GRID:grid.15895.30) (ISNI:0000 0001 0738 8966) 
8  University of Gothenburg, Wallenberg Centre for Molecular and Translational Medicine, Gothenburg, Sweden (GRID:grid.8761.8) (ISNI:0000 0000 9919 9582); Institute of Physiology and Neuroscience, University of Gothenburg, Department of Psychiatry and Neurochemistry, Gothenburg, Sweden (GRID:grid.8761.8) (ISNI:0000 0000 9919 9582); Institute of Neurology, University College London, Dementia Research Centre, London, UK (GRID:grid.83440.3b) (ISNI:0000000121901201) 
9  Wallenberg Laboratory, Institute of Medicine, University of Gothenburg, Department of Molecular and Clinical Medicine, Gothenburg, Sweden (GRID:grid.8761.8) (ISNI:0000 0000 9919 9582) 
10  University of California Davis, NIH West Coast Metabolomics Center, Genome Center, Davis, USA (GRID:grid.27860.3b) (ISNI:0000 0004 1936 9684); University of California Davis, Department of Nutrition, Davis, USA (GRID:grid.27860.3b) (ISNI:0000 0004 1936 9684); USDA, ARS, Western Human Nutrition Research Center, Davis, USA (GRID:grid.507310.0) 
11  Institute of Clinical Sciences, Sahlgrenska University Hospital, University of Gothenburg, Department of Gastrosurgical Research and Education, Gothenburg, Sweden (GRID:grid.8761.8) 
12  Wallenberg Laboratory, Institute of Medicine, University of Gothenburg, Department of Molecular and Clinical Medicine, Gothenburg, Sweden (GRID:grid.8761.8) (ISNI:0000 0000 9919 9582); School of Medicine, University of California San Diego, Division of Cardiology, San Diego, USA (GRID:grid.266100.3) (ISNI:0000 0001 2107 4242) 
13  Wallenberg Laboratory, Institute of Medicine, University of Gothenburg, Department of Molecular and Clinical Medicine, Gothenburg, Sweden (GRID:grid.8761.8) (ISNI:0000 0000 9919 9582); University of Gothenburg, Wallenberg Centre for Molecular and Translational Medicine, Gothenburg, Sweden (GRID:grid.8761.8) (ISNI:0000 0000 9919 9582); Sahlgrenska University Hospital, Department of Clinical Physiology, Gothenburg, Sweden (GRID:grid.1649.a) (ISNI:000000009445082X) 
Publication year
2019
Publication date
2019
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-019-51673-0
ProQuest document ID
2309778212
Copyright
© The Author(s) 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.