Abstract

State-of-the-art ultra-sensitive blood glucose-monitoring biosensors, based on glucose oxidase (GOx) covalently linked to a single layer graphene (SLG), will be a valuable next generation diagnostic tool for personal glycemic level management. We report here our observations of sensor matrix structure obtained using a multi-physics approach towards analysis of small-angle neutron scattering (SANS) on graphene-based biosensor functionalized with GOx under different pH conditions for various hierarchical GOx assemblies within SLG. We developed a methodology to separately extract the average shape of GOx molecules within the hierarchical assemblies. The modeling is able to resolve differences in the average GOx dimer structure and shows that treatment under different pH conditions lead to differences within the GOx at the dimer contact region with SLG. The coupling of different analysis methods and modeling approaches we developed in this study provides a universal approach to obtain detailed structural quantifications, for establishing robust structure-property relationships. This is an essential step to obtain an insight into the structure and function of the GOx-SLG interface for optimizing sensor performance.

Details

Title
Structural determination of Enzyme-Graphene Nanocomposite Sensor Material
Author
Rai, Durgesh K 1 ; Manickam Gurusaran 2 ; Urban, Volker 3   VIAFID ORCID Logo  ; Aran, Kiana 4 ; Ma, Lulu 5 ; Li, Pingzuo 6 ; Qian, Shuo 3   VIAFID ORCID Logo  ; Narayanan, Tharangattu N 7 ; Ajayan, Pulickel M 5   VIAFID ORCID Logo  ; Liepmann, Dorian 4   VIAFID ORCID Logo  ; Sekar, Kanagaraj 8 ; Álvarez-Cao, María-Efigenia 9 ; Escuder-Rodríguez, Juan-José 9 ; María-Esperanza Cerdán 9 ; González-Siso, María-Isabel 9   VIAFID ORCID Logo  ; Viswanathan, Sowmya 10 ; Ramasamy Paulmurugan 11 ; Venkatesan Renugopalakrishnan 12 

1  Cornell High Energy Synchrotron Source, Cornell University, Ithaca, New York, USA 
2  Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne-NE1 7RU, UK 
3  Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA 
4  Department of Bioengineering, University of California, Berkeley, Berkeley, California, USA 
5  Department of Mechanical Engineering and Materials Science, Rice University, Houston, Texas, USA 
6  Center for Life Sciences, Boston Children’s Hospital, Harvard Medical School, Boston, Massachusetts, USA 
7  Tata Institute of Fundamental Research – Center for Interdisciplinary Sciences, Hyderabad, India 
8  Department of Computational and Data Sciences, Indian Institute of Science, Bangalore, India 
9  Universidade da Coruña, Grupo EXPRELA, F. Ciencias & Centro de Investigacións Científicas Avanzadas (CICA) & Instituto de Investigación Biomédica A Coruña (INIBIC), A Coruña, Spain 
10  Newton Wellesley Hospital/Partners Healthcare System, Newton, Massachusetts, USA 
11  Cellular Pathway Imaging Laboratory (CPIL), Dept. of Radiology, Stanford University School of Medicine, 3155 Porter Drive, Suite 2236, Palo Alto, California, USA 
12  Center for Life Sciences, Boston Children’s Hospital, Harvard Medical School, Boston, Massachusetts, USA; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA 
Pages
1-11
Publication year
2019
Publication date
Oct 2019
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-019-51882-7
ProQuest document ID
2310420993
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.