Abstract

Graphene, owing to its inherent chemical inertness, biocompatibility, and mechanical flexibility, has great potential in guiding cell behaviors such as adhesion and differentiation. However, due to the two-dimensional (2D) nature of graphene, the microfabrication of graphene into micro/nanoscale patterns has been widely adopted for guiding cellular assembly. In this study, we report crumpled graphene, i.e., monolithically defined graphene with a nanoscale wavy surface texture, as a tissue engineering platform that can efficiently promote aligned C2C12 mouse myoblast cell differentiation. We imparted out-of-plane, nanoscale crumpled morphologies to flat graphene via compressive strain-induced deformation. When C2C12 mouse myoblast cells were seeded on the uniaxially crumpled graphene, not only were the alignment and elongation promoted at a single-cell level but also the differentiation and maturation of myotubes were enhanced compared to that on flat graphene. These results demonstrate the utility of the crumpled graphene platform for tissue engineering and regenerative medicine for skeletal muscle tissues.

Details

Title
Uniaxially crumpled graphene as a platform for guided myotube formation
Author
Kim, Junghoon 1 ; Leem, Juyoung 1   VIAFID ORCID Logo  ; Hong Nam Kim 2 ; Kang, Pilgyu 3 ; Choi, Jonghyun 1 ; Haque, Md Farhadul 1 ; Kang, Daeshik 4   VIAFID ORCID Logo  ; Nam, SungWoo 5   VIAFID ORCID Logo 

 Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA 
 Center for BioMicrosystems, Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea; Division of Bio-Medical Science and Technology, KIST School, Korea University of Science and Technology, Seoul, Republic of Korea 
 Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA; Department of Mechanical Engineering, George Mason University, Fairfax, VA, USA 
 Department of Mechanical Engineering, Ajou University, Suwon, Republic of Korea 
 Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA; Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA 
Pages
1-10
Publication year
2019
Publication date
Nov 2019
Publisher
Springer Nature B.V.
ISSN
20961030
e-ISSN
20557434
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2311583279
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.