Abstract

The Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. Both proteins are homologues of the yeast ARP2/3 complex activator, Pan1p. Here, we show that these proteins are also involved in actin cytoskeleton regulated autophagy. Both AtEH/Pan1 proteins localise to the plasma membrane and autophagosomes. Upon induction of autophagy, AtEH/Pan1 proteins recruit TPC and AP-2 subunits, clathrin, actin and ARP2/3 proteins to autophagosomes. Increased expression of AtEH/Pan1 proteins boosts autophagosome formation, suggesting independent and redundant pathways for actin-mediated autophagy in plants. Moreover, AtEHs/Pan1-regulated autophagosomes associate with ER-PM contact sites (EPCS) where AtEH1/Pan1 interacts with VAP27-1. Knock-down expression of either AtEH1/Pan1 or VAP27-1 makes plants more susceptible to nutrient depleted conditions, indicating that the autophagy pathway is perturbed. In conclusion, we identify the existence of an autophagy-dependent pathway in plants to degrade endocytic components, starting at the EPCS through the interaction among AtEH/Pan1, actin cytoskeleton and the EPCS resident protein VAP27-1.

Details

Title
Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery
Author
Wang, Pengwei 1 ; Pleskot, Roman 2 ; Zang, Jingze 1 ; Winkler, Joanna 2 ; Wang, Jie 3 ; Yperman, Klaas 2 ; Zhang, Tong 4 ; Wang, Kun 4 ; Gong, Jinli 4 ; Guan, Yajie 4 ; Richardson, Christine 5   VIAFID ORCID Logo  ; Duckney, Patrick 5 ; Vandorpe, Michael 2 ; Mylle, Evelien 2 ; Jindriska Fiserova 6 ; Daniel Van Damme 2 ; Hussey, Patrick J 5 

1  Department of Biosciences, Durham University, Durham, UK; Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei Province, PR China 
2  Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium; VIB Center for Plant Systems Biology, Ghent, Belgium 
3  Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium 
4  Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei Province, PR China 
5  Department of Biosciences, Durham University, Durham, UK 
6  Department of Biosciences, Durham University, Durham, UK; Department of Biology of the Cell Nucleus, Institute of Molecular Genetics CAS, Prague, Czech Republic 
Pages
1-16
Publication year
2019
Publication date
Nov 2019
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-019-12782-6
ProQuest document ID
2314311398
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.