Abstract

The Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. Both proteins are homologues of the yeast ARP2/3 complex activator, Pan1p. Here, we show that these proteins are also involved in actin cytoskeleton regulated autophagy. Both AtEH/Pan1 proteins localise to the plasma membrane and autophagosomes. Upon induction of autophagy, AtEH/Pan1 proteins recruit TPC and AP-2 subunits, clathrin, actin and ARP2/3 proteins to autophagosomes. Increased expression of AtEH/Pan1 proteins boosts autophagosome formation, suggesting independent and redundant pathways for actin-mediated autophagy in plants. Moreover, AtEHs/Pan1-regulated autophagosomes associate with ER-PM contact sites (EPCS) where AtEH1/Pan1 interacts with VAP27-1. Knock-down expression of either AtEH1/Pan1 or VAP27-1 makes plants more susceptible to nutrient depleted conditions, indicating that the autophagy pathway is perturbed. In conclusion, we identify the existence of an autophagy-dependent pathway in plants to degrade endocytic components, starting at the EPCS through the interaction among AtEH/Pan1, actin cytoskeleton and the EPCS resident protein VAP27-1.

Details

Title
Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery
Author
Wang, Pengwei 1 ; Pleskot, Roman 2 ; Zang, Jingze 1 ; Winkler, Joanna 2 ; Wang, Jie 3 ; Yperman, Klaas 2 ; Zhang, Tong 4 ; Wang, Kun 4 ; Gong, Jinli 4 ; Guan, Yajie 4 ; Richardson, Christine 5   VIAFID ORCID Logo  ; Duckney, Patrick 5 ; Vandorpe, Michael 2 ; Mylle, Evelien 2 ; Jindriska Fiserova 6 ; Daniel Van Damme 2 ; Hussey, Patrick J 5 

 Department of Biosciences, Durham University, Durham, UK; Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei Province, PR China 
 Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium; VIB Center for Plant Systems Biology, Ghent, Belgium 
 Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium 
 Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei Province, PR China 
 Department of Biosciences, Durham University, Durham, UK 
 Department of Biosciences, Durham University, Durham, UK; Department of Biology of the Cell Nucleus, Institute of Molecular Genetics CAS, Prague, Czech Republic 
Pages
1-16
Publication year
2019
Publication date
Nov 2019
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2314311398
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.